摘要
为了解中国农业科学院特产研究所分子生物学重点实验室前期分离的牛病毒性腹泻病毒BVDV-JL毒株完整的基因序列信息,本试验对BVDV-JL F6代毒株进行了完整基因序列测定。利用反转录—聚合酶链式反应(RT-PCR)方法分段扩增了BVDV-JL分离毒株的7段cDNA片段,分别克隆于pMD18-T载体并进行测序。BVDV-JL株基因组序列全长为12276bp,编码3901个氨基酸。基因组两端为非编码区5′UTR和3′UTR。基因比对及进化树分析结果表明BVDV-JL与BVDV CP7同源性最高,核苷酸同源性为93.2%,归类于BVDV-1b2基因亚型。BVDV-JL是第1个在中国报道的BVDV-1b2亚型毒株。了解BVDV-JL完整基因序列有利于中国BVDV流行病学调查。
The bovine viral diarrhea virus（BVDV）JL strain was isolated from a cattle showing clinical diarrhea signs in Jilin province.In order to analyze the complete genome of BVDV-JL,7fragments of BVDV-JL were amplified by RT-PCR and cloned into pMD18-T vector then sent for sequencing by commercial serves.Complete genome of the virus was 12276 bp in length and contains a 5′UTR,one open reading frame encoding apolyprotein of 3901 amino acids and a 3′UTR.The results of genomic comparison and phylogenetic analysis showed that BVDV-JL fell into BVDV-1b2 subtype and was most similar to CP7,the nucleotide was 93.2%.BVDV-JL was the first reported BVDV-1b2 strains,BVDV-JL complete genomic sequences enriched the available genomic sequences and it would be benefit to epidemiological surveys of BVDV detected in China.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第10期23-27,共5页
China Animal Husbandry & Veterinary Medicine
基金
中央级公益性科研院所基本科研业务费(00320-14016)
关键词
牛病毒性腹泻病毒
全基因组
进化树
bovine viral diarrhea virus
complete genome
phylogeny
