奶牛乳房炎金黄色葡萄球菌8种毒力基因的PCR检测

Detection of the Main 8 Virulence Genes of Staphylococcus aureus from Bovine Mastitis

为研究奶牛乳房炎金黄色葡萄球菌(Staphylococcus aureus,SA)主要毒力因子的分布情况,本试验利用PCR对70株临床型乳房炎、55株隐性乳房炎金黄色葡萄球菌的8种主要毒力基因进行检测。结果显示,nuc、ClfA、TSST-1、PVL、Hla、Hlb、FnBPA和FnBPB8种毒力基因在临床型乳房炎SA菌株中的检出率分别为:100.0%(70株)、100.0%(70株)、0(0株)、5.7%(4株)、100.0%(70株)、11.4%(8株)、97.1%(68株)和100.0%(70株);在隐性乳房炎SA菌株中的检出率分别为:100.0%(55株)、100.0%(55株)、0(0株)、70.9%(39株)、98.2%(54株)、9.1%(5株)、100.0%(55株)和100.0%(55株)。结果表明,nuc、ClfA、Hla、Hlb和FnBPA5种毒力基因是引起奶牛乳房炎的SA的最主要毒力基因;PVL基因可能是引起隐性乳房炎的重要致病基因。

The aim of the present study was to investigate the main virulence genes properties of Staphylococcus aureus（SA）strains from bovine mastitis.For this purpose,8kinds of virulence genes were detected from 70 isolates of clinical mastitis and 55 isolates of subclinical mastitis with PCR.The results were as follows,the detecting rates of nuc,ClfA,TSST-1,PVL,Hla,Hlb,FnBPA and FnBPB were 100.0%（70isolates）,100.0%（70isolates）,0（0 isolate）,5.7%（4isolates）,100.0%（70isolates）,11.4%（8isolates）,97.1%（68isolates）and 100.0%（70 isolates）respectively in clinical mastitis;whereas 100.0%（55isolates）,100.0%（55isolates）,0（0isolate）,70.9%（39isolates）,98.2%（54isolates）,9.1%（5isolates）,100.0%（55isolates）and 100.0%（55isolates）in subclinical mastitis.The results implied that nuc,ClfA,Hla,Hlb and FnBPA were the main virulence genes in SA isolates both in clinical mastitis and subclinical mastitis.PVLgene might be a key pathogenic gene for the cows with subclinical mastitis.