1例藏香猪伪狂犬病、猪圆环病毒病与附红细胞体病混合感染的诊治

Diagnosis and Treatment of Porcine Pseudorabies,Porcine Circovirus Disease Mixed Infections with Porcine Eperythrozoonosis in a Tibetan Pig

为对送检的发病藏香猪病死因进行确诊,本试验采用常规PCR、RT-PCR及荧光定量PCR方法,并结合流行病学调查、临床诊断及病理剖检等实验室检测对送检病料进行诊断,结果显示病死猪心脏、肺脏充血出血,肺脏肉变,气管内充满白色泡沫,全身淋巴结出血;荧光定量PCR方法检测猪圆环病毒呈阳性,PCR方法检出猪伪狂犬病病毒特异性条带,未见猪传染性胸膜肺炎放线杆菌、猪肺炎支原体条带,RT-PCR方法未扩增出猪瘟病毒、猪繁殖与呼吸综合征病毒特异性条带;血液涂片染色镜检可见猪附红细胞体。结果表明病死猪为猪伪狂犬病、猪圆环病毒病与猪附红细胞体病混合感染,采用经实验室诊断给出的综合防治方案治疗后,疫情得到控制。本次病例的诊治为养猪业可能发生的猪伪狂犬病、猪圆环病毒病、猪附红细胞体病的混合感染提供了有效的防治方法和借鉴经验。

In order to diagnose the cause of disease and death of Tibetan pigs,PCR,RT-PCR and Real-time PCR methods were used in this experiment combined with epidemiological investigation,clinical diagnosis,autopsy pathology and other laboratory tests,the results showed that congestion and hemorrhage of heart and lung,lung became meat,trachea filled with white foam,systemic lymph hemorrhage of Tibetan pig,Real-time PCR detected porcine circovirus type 2positive,PCR detected porcine pseudorabies virus positive,there was no Pleuropneumoniae actinobacillus and Mycoplasma hyopneumoniae strips,RT-PCR did not amplify the strips of classical swine fever virus and porcine reproductive and respiratory syndrome virus,staining microscopy showed there was Mycoplasma suis.The results showed the diseased pig was mixed infections with porcine pseudorabies,porcine circovirus disease and porcine eperythrozoonosis,after integrated control,the epidemic situation had been controlled.Diagnosis and treatment of the case might provide effective prevention and treatment methods and experiences for mixed infections by porcine pseudorabies,porcine circovirus disease and porcine eperythrozoonosis.