LTA4H基因多态性及吸烟对动脉粥样硬化型脑梗死发生影响

INFLUENCE OF LTA4H GENE POLYMORPHISM AND SMOKING ON ATHEROSCLEROTIC CEREBRAL INFARCTION

目的探讨白三烯A4水解酶（LTA4H）基因多态性及吸烟对动脉粥样硬化型脑梗死（ACI）发生的影响。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法,对450例ACI病人和400例非脑卒中者进行LTA4Hrs6538697T/C位点基因型检测。采用Logistic回归分析该位点多态性与ACI发病风险的相关性,以及基因多态性与吸烟共同作用对ACI发生的影响。结果经Logistic回归方法排除脑卒中的传统危险因素影响后,LTA4Hrs6538697C等位基因携带者ACI易感性是非携带者的1.34倍（OR=1.34,95%CI=1.010-1.786,P〈0.05）,吸烟者ACI的发病风险是非吸烟者的1.97倍（OR=1.97,95%CI=1.306-2.733,P〈0.05）,携带LTA4Hrs6538697C等位基因同时为吸烟者的人群患ACI风险是非携带C等位基因吸烟人群的3.07倍（OR=3.07,95%CI=1.489-6.340,P〈0.05）。结论 LTA4Hrs6538697C等位基因的高表达可增加ACI的发病风险,LTA4Hrs6538697基因多态性与吸烟在ACI的发生中存在协同作用。

Objective To explore the association between polymorphism of leukotriene A4hydrolase（LTA4H）gene and smoking on atherosclerotic cerebral infarction（ACI）. Methods The LTA4Hrs6538697 gene of 450 ACI patients and 400 subjects without ACI was genotyped by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）method,to explore the interaction between polymorphism of LTA4 Hgene and smoking on ACI. Results Multivariate Logistic regression was used to adjust for traditional risk factors,carriers of rs6538697 Callele were more susceptible to ACI（OR=1.34,95%CI=1.010-1.786,P〈0.05）,smoking could increase the risk of ACI about 1.97times（OR=1.97,95%CI=1.306-2.733,P〈0.05）,carriers of rs6538697 Callele who were smokers at the same time showed significantly increased risk of ACI（OR=3.07,95%CI=1.489-6.340,P〈0.05）. Conclusion The high expression of LTA4Hrs6538697 Ccan increase the risk of ACI,both LTA4Hrs6538697 Cand smoking play ajoint action in the development of ACI.