结直肠癌病人肠道黏膜菌群结构的变化

CHANGES OF STRUCTURE IN INTESTINAL MICROFLORA IN PATIENTS WITH COLORECTAL CANCER

目的研究结直肠癌（CRC）病人肠道黏膜菌群结构的变化。方法选取50例CRC病人的癌组织和癌旁正常组织作为研究对象,以454焦磷酸测序方法检测16SrRNA基因V3-V5区,研究CRC病人肿瘤组织的菌群结构并与癌旁正常组织进行比较。结果 CRC病人癌组织中的梭杆菌属、弯曲菌属丰度与癌旁正常组织相比明显降低,Faecalibacterium、罗氏菌属丰度明显升高,差异有显著性（Z=1.84~3.63,P〈0.05）。结论 CRC病人癌组织菌群较周围正常组织发生了明显改变,出现了有害的条件致病菌增加、有益的细菌减少的结构失调现象。肠道黏膜菌群结构鉴定可能成为诊断CRC高风险人群的重要途径,干预和调节肠道菌群可能成为预防和治疗CRC的新方法。

Objective To observe the changes of structure of intestinal microflora in patients with colorectal cancer （CRC）. Methods CRC tissue and paraneoplastie normal tissue were taken from 50 CRC patients in the present study. The V3 V5 region of 16S rRNA gene was detected using 454 pyrosequencing to investigate the structure of flora in cancer tissue and compare with that in paraneoplastic normal tissue. Results The abundance of Fusobacterium and Campylobacter in cancer tissue declined and Faecalibacteriurn and Roseburia elevated versus that in paraneoplastic normal tissue, the differences being significant （Z=1.84--3.63,P〈0.05）. Conclusion The structure of mieroflora in cancer tissue of patients with eolorectal cancer is obviously changed as compared with its surrounding normal tissue, emerging structural imbalance such as harmful opportunistic pathogens increase, and beneficial bacteria decrease. The identification of structure of intestinal flora may become a significant way in the diagnosis of high risk population of this malignacies, interference and regulation of the flora is likely to become a new method in the prevention and therapy of this disease.