恒河猴肝移植模型急性排斥反应中白细胞介素6的表达

Interleukin-6 expression in rhesus monkey models with acute rejection after liver transplantation

背景:白细胞介素6是免疫炎症反应中一种重要的细胞因子,它与移植排斥反应具有密切的关系,在移植排斥反应诊断和抗排斥疗效评价中发挥着重要作用。目的:检测白细胞介素6在恒河猴肝移植后急性排斥反应中的表达水平,以评价其作为肝移植后急性排斥反应早期诊断指标的价值。方法:以16只恒河猴为研究对象,将其随机分为实验组(围手术期不给予免疫抑制治疗)和对照组(围手术期给予免疫抑制治疗),然后建立恒河猴同种异体原位肝移植模型;分别在移植后6,12,24,72 h时间点收集血清及肝脏组织,通过检测肝功指标和移植肝组织苏木精-伊红染色Banff评分反映其移植排斥反应情况,并应用酶联免疫吸附法、免疫组织化学技术分别检测白细胞介素6的表达水平。结果与结论:肝移植后12,24,72 h实验组均有急性排斥反应发生,其中实验组丙氨酸氨基转移酶、天冬氨酸氨基转移酶、总胆红素在24 h和72 h表达水平明显高于对照组(P<0.05);实验组72 h组织学表现重于对照组,Banff评分高于对照组(P<0.05);实验组中血清、肝组织的白细胞介素6水平在肝移植后12,24,72 h明显高于对照组(P<0.05),并以72 h最为明显。结果表明白细胞介素6可能参与肝移植后排斥反应的发生,其表达可能在恒河猴原位肝移植后急性排斥反应的早期诊断中有一定意义。

BACKGROUND：Interleukin-6 is an important cytokine in the immune inflammatory response, strongly links with graft rejection reaction, and plays an important role in diagnosis of graft rejection and evaluation of anti-rejection. OBJECTIVE：To measure the expression of interleukin-6 in acute rejection of the liver transplantation in the rhesus monkey, and to evaluate the value as an early diagnosis of acute rejection after liver transplantation. METHODS：A total of 16 rhesus monkeys were used as the object and randomly divided into experimental group （no treated by immunosuppressant in perioperative period）, and control group （treated by immunosuppressant in perioperative period）. The al ograft orthotopic liver transplantation models were established in those monkeys. Then serum and liver tissue were col ected at 6, 12, 24, and 72 hours after surgery. Al ograft rejection was monitored by liver function tests, and hematoxylin-eosin staining of liver and Banff score. Final y, the expression levels of interleukin-6 were detected by enzyme linked immunosorbent assay and immunohistochemistry.RESULTS AND CONCLUSION：Acute graft rejection reaction appeared at 12, 24 and 72 hours after liver transplantation in the experimental group. The expressions of alanine aminotransferase, aspartate aminotransferase and total bilirubin were significantly higher in the experimental group than in the control group at 24 and 72 hours （P〈0.05）. Histological manifestations were severer and Banff score was higher in the experimental group at 72 hours than in the control group （P〈0.05）. Interleukin-6 levels were significantly higher in the serum and liver tissue of experimental group than in the control group at 12, 24 and 72 hours after liver transplantation （P〈0.05）, especial y at 72 hours. Results suggested that interleukin-6 possibly participated in rejection after liver transplantation. The expression of interleukin-6 was probably of significance in the early diagnosis of acute rejection after orthotopic liver transplantation in rhesus monkeys.