白细胞介素1β和白细胞介素17在根尖周病组织肥大细胞上的表达

Expression of IL-1β and IL-17 on mast cells in human periapical diseases

目的:观察不同类型慢性根尖周病组织中肥大细胞(mast cells,MCs)的分布情况,并分析白细胞介素1β(interleukin-1β,IL-1β)和白细胞介素17(interleukin-17,IL-17)在MCs上的表达情况,探讨MCs IL-1β和IL-17在根尖周病发病机制中的作用。方法:本实验共收集102例样本,分为3组:(1)根尖肉芽肿组32例;(2)根尖囊肿组35例;(3)正常对照组35例。组织标本经4%中性甲醛缓冲液固定48 h以上;石蜡包埋,组织连续切片,HE染色,在光学显微镜下观察其组织学改变;采用免疫荧光双染色法,在荧光显微镜下观察各组标本组织表达IL-1β和IL-17的MCs数目。结果:两组慢性根尖周病的炎症反应程度明显高于正常对照组(P<0.01);根尖囊肿组与根尖肉芽肿组之间炎症反应程度无显著差异(P>0.05)。与正常对照组相比,两组慢性根尖周病组织中IL-1β和IL-17阳性MCs数目均显著增多(P<0.01);根尖囊肿组与根尖肉芽肿组IL-1β和IL-17阳性MCs密度无显著性差异(P>0.05)。经Pearson相关分析,3组标本组织中IL-1β和IL-17阳性MCs密度与组织炎症反应程度存在直线的正相关关系(P<0.01)。结论:慢性根尖周病组织中MCs数目明显增多,同时IL-1β和IL-17阳性的MCs密度显著增高。IL-1β和IL-17阳性MCs密度与根尖周病的炎症程度趋势相一致。这提示IL-1β和IL-17阳性MCs可能在慢性根尖周病的致病机制中发挥重要作用。

AIM： To identify and quantify the expression of IL-1β and IL-17 in mast cells（ MCs） in different types of human pericapical diseases using double immunofluorescence staining. METHODS： The specimens（ n = 102）,including healthy control（ n = 35）,periapical cyst（ n = 35） and periapical granuloma（ n = 32）,were involved in the present study. The tissue samples were fixed in 10% buffered formalin for at least 48 h and then embedded in paraffin.Serial 5-μm-thick sections were deposited onto SuperFrost /Plus microscope glasses. Routine staining of the sections using hematoxylin eosin（ HE） was performed for morphological evaluation. The number of IL-1β and IL-17 positive MCs was identified by double immunofluorescence staining. RESULTS： Compared with the healthy controls,the inflammation score of periapical lesions was significantly increased in the periapical patients（ P 〈0. 01）. The density of IL-1β and IL-17 positive MCs in the periapical lesions were obviously higher than that in the healthy controls（ P〈 0. 01）. However,no significant difference between periapical cyst and periapical granuloma was observed. The Pearson correlation analysis showed that there was a positive correlation between the density of IL-1β and IL-17 double positive MCs and inflammation score in different groups of specimens（ P 〈0. 01）. CONCLUSION： There is significantly increased number of MCs,along with increased density of IL-1β and IL-17 positive MCs in human periapical lesions. The increased density of IL-1β and IL-17 positive MCs has the similar tendency as the severity of tissue inflammation in human periapical lesions,suggesting that IL-1β and IL-17 positive MCs may play an important role in the pathogenesis of human periapical diseases.