Y盒结合蛋白1在SK-BR-3乳腺癌细胞增殖中的作用

Roles of Y box-binding protein 1 in SK-BR-3 breast cancer proliferation

目的探讨Y盒结合蛋白1（YBl）在乳腺癌细胞增殖中的作用。方法收集河北大学附属医院2013年6-8月的乳腺浸润性导管癌及癌旁正常乳腺组织标本，实时定量PCR（qRT-PCR）分析YBl基因的mRNA水平；培养乳腺上皮细胞HBL-100和乳腺癌细胞MCF-7、MDA-MB-231和SK-BR-3，qRT-PCR观察YBl的表达水平，MTS法分析细胞增殖活力；血小板源性生长因子BB（PDGF-BB）刺激SK-BR-3细胞，观察YBl的表达水平；应用特异性小干扰RNA敲低内源性YBl或应用GFP-YBl腺病毒载体过表达外源性GFP-YBl，MTS法观察敲低或过表达YBl对细胞增殖的影响。结果与癌旁正常乳腺组织相比，乳腺癌组织中YBl表达水平显著增高（P〈0．05），增殖能力较强的MDA-MB-231和SK．BR．3乳腺癌细胞株中YBl的表达水平显著高于乳腺上皮细胞HBL-100（P〈0．05）。PDGF．BB可上调SK-BR-3细胞中YBl表达水平。敲低内源性YBl可抑制SK-BR-3细胞增殖，而过表达外源性YBl可促进SK-BR-3细胞增殖活性。结论YBl在乳腺癌组织中表达升高并促进乳腺癌细胞增殖。

Objective To explore the roles of Y box-binding protein 1 （YB-1） in breast cancer cell proliferation. Methods Twenty cases of surgical removal of breast cancer tissue （diagnosed with invasive ductal carcinoma, stage Ⅱ, by postoperative paraffin pathology） and normal breast tissues adjacent to carcinoma were collected during June 2013 to August 2013. Quantitative real-time PCR （qRT-PCR） was performed to detect the YB1 mRNA levels. Cultured mammary epithelial cells （HBL-100） and breast cancer cells （MCFT, MDA-MB-231 ＆ SK-BR-3 cells） were harvested and qRT-PCR was performed to detect the YB1 mRNA levels. SK-BR-3 cells were stimulated with various concentrations of PDGF-BB and YB1 expression levels were detected by qRT-PCR. Down-regulation or over-expression of YB1 by si-YB1 or Ad- GFP-YB1 was detected in SK-BR-3 ceils. And MTS cell proliferation assay kit was used to detect cell proliferation. Results YB1 mRNA levels were significantly higher in breast cancer tissues and MDA-MB- 231 and SK-BR-3 breast cancer cell lines than that in adjacent normal breast tissues and HBL-100 mammary epithelial cells respectively （ P 〈 0. 05 ）. YB1 expression levels increased in PDGF-BB stimulated SK-BR-3 cells in a dose-dependent manner. Conclusion A down-regulation of endogenous YB1 decreases and an over-expression of exogenous YB1 promotes the proliferation activity in SK-BR-3 cells.