转Bt基因水稻对白符跳虫取食选择行为的影响

Tests of the preference of Folsomia candida for isogenic and Bt-rice

【目的】为探讨转基因Bt水稻种植对土壤动物的潜在生态风险性。【方法】本研究将3种转Bt基因水稻及其非转基因亲本水稻叶片残体饲养白符跳虫Folsomia candida,通过观察其粪便的数量与分布以分析白符跳虫对Bt水稻的取食选择行为。【结果】研究结果表明,Bt蛋白(Cry1Ab和Cry1Ac)不会影响白符跳虫的取食选择;而Bt基因插入后导致的水稻成分的变化可能影响了白符跳虫对水稻残体的偏好性。结果可为评估转Bt水稻对土壤生态系统影响提供参考价值,为转Bt水稻安全性评价提供科学的依据。

[Objectives] To evaluate the ecological risk of transgenic Bt rice on soil animals. [Methods] We fed three different varieties of transgenic Bt rice and their isogenic rice tissue residues to Folsomia candida and assessed the feeding preferences of F. candida by determining the quantity and distribution of fecal pellets associated with different Bt or non-Bt rice. [Results] The Bt protein （CrylAb and CrylAc） of transgenic Bt rice had little impact on feeding intensity. However, inserting the Bt gene into rice plants changes the chemical composition of rice, which may affect the preferences ofF. candida for plant tissue residues. These results provide a reference for the impact of transgenic Bt rice on the soil system, and provide a scientific basis for estimating the security of transgenic Bt rice.