转Bt基因水稻对白符跳虫适应低温环境与排泄的影响

Low temperature adaption experiments on Folsomia candida fed isogenic and Bt-rice

【目的】为探讨转基因Bt水稻种植对土壤重要分解者跳虫的潜在生态风险性。【方法】将同一非转基因亲本插入3种不同的Bt基因(cry1C、cry2A、cry1Ab/Ac)的转Bt基因水稻叶片残体饲养白符跳虫Folsomia candida,通过放入不同低温环境下观察其存活率和粪便排泄速率以分析白符跳虫取食Bt水稻叶片后的适应低温行为。【结果】结果表明,3种转Bt基因水稻相对于非转基因亲本水稻品种而言,不会影响白符跳虫的适应低温环境;而不同Bt基因插入后导致的水稻成分的变化可能影响了白符跳虫对水稻残体的偏好性,进而影响其在低温环境下的适应行为。【结论】结果可为评估转Bt水稻对土壤生态系统影响提供参考价值,为转Bt水稻安全性评价提供科学的依据。

[Objectives] To evaluate the potential ecological risk of transgenic Bt rice to ground-dwelling arthropods. [Methods] Three different transgenic Bt rice varieties and their isogenic rice tissue residues were fed to Folsomia candida for 28 days after which these were exposed to low temperature to assess their low temperature adaption on the basis of survival and excretion. [Results] The results show that being fed transgenic Bt rice had no effect on low temperature adaption compared to isogenic rice. However, inserting the Bt gene into rice plants could change the chemical composition of rice, which might affect the survival and excretion ofF. candida at low temperature. [Conclusion] This work is a case study for evaluating the impact of the transgenic Bt rice on the soil ecosystem, and also provides a scientific basis for estimating the safety oftransgenic Bt rice.