红凤菜新鲜茎叶中总黄酮提取物的LC-MS分析

LC-MS analysis of total flavonoids extracts from fresh stem and leaf of Gynura bicolor

红凤菜（Gynura bicolor DC.）为菊科（Compositae）菊三七属（Gynura Cass.）植物，别名血皮菜、观音菜、观音苋、紫背天葵等，主要分布于中国南方各地，全草均可入药[1]；也可作为蔬菜食用，在国内多个地区均有栽培和销售。目前已知红凤菜含有黄酮类、酚性酸类、萜类、甾醇类、脂肪酸类、生物碱类及花青素类等[2-7]成分。由于黄酮类成分多具有保护心血管、抗肿瘤、抗糖尿病、抗氧化、抗炎和抗病毒等作用[8]，因此对红凤菜中黄酮类成分的研究具有深度开发价值。

Four total flavonoids extracts were obtained from fresh stem and leaf of Gynura bicolor DC. by method of ethanol extraction-macroporous resin column chromatography-polyamide column chromatography-recrystallization, and their compositions were analyzed by LC-MS technology. The results show that there are six groups of flavonoid absorption peak in total flavonoids extracts of G. bicolor, which is identified to be eight components including rutin, quercetin-3-O- rubinobioside, hyperoside, isoquercitrin, kaempferol-3-O-rubinobioside, kaempferol-3-O-rutinoside, kaempferol-3-O- galactoside and astragalin. This extraction method can effectively extract and purify total flavonoids from fresh stem and leaf of G. bicolor, so it can be used for factory production.