细胞膜片技术在组织工程和再生医学领域的应用

Research Progress of Cell Sheet Technology and Its Applications in Tissue Engineering and Regenerative Medicine

细胞膜片技术是通过物理的方法将扩增融合的细胞从培养皿底壁分离,获得膜片状细胞结构的一种技术。与传统胰酶消化收集细胞的方法相比,运用该技术收集到的细胞保留了体外培养过程中分泌的胞外基质、建立的细胞-基质连接以及细胞间连接等结构。本文综述了细胞膜片技术在构建包括软组织(角膜、黏膜、心肌、血管、胰岛、肝组织、膀胱、皮肤)和硬组织(骨、软骨、牙根)等组织再生领域的相关应用,以期为组织工程和再生医学的发展提供新思路,并提出今后可在提高该技术的操作性及构建大块组织可行性方面进行深入研究。

Cell sheet engineering is an important technology to harvest the cultured cells in the form of confluent mon- olayers using a continuous culture method and a physical approach. Avoiding the use of enzymes, expended ceils can be harvested together with endogenous extracellular matrix, cell-matrix contacts, and cell-cell contacts. With high efficiency of cell loading ability and without using exogenous scaffolds, cell sheet engineering has several advantages over traditional tissue engineering methods. In this article, we give an overview on cell sheet technology about its ap- plications in the filed of tissue regeneration, including the construction of soft tissues （corneal, mucous membrane, myocardium, blood vessel, pancreas islet, liver, bladder and skin） and hard tissues （bone, cartilage and tooth root）. This techonoly is promising to provide a novel strategy for the development of tissue engineering and regenerative medicine. And further works should be carried out on the operability of this technology and its feasibility to construct thick tissues.