人外周血内皮细胞与大鼠胰岛共移植改善胰岛的存活和功能

Improve The Survival and Function of Islets by Combined Transplantation of Human Endothelial Cells in Peripheral Blood and Islet of Rats

目的探讨人外周血内皮细胞与大鼠胰岛共移植到糖尿病裸鼠肾包膜下改善移植胰岛存活及其功能发挥情况。方法将人外周血内皮细胞(5×105个)与新鲜分离的大鼠胰岛细胞共移植到糖尿病裸鼠模型肾包膜下,检测移植受体裸鼠空腹血糖水平、体质量和外周血C肽水平,并行腹腔葡萄糖耐量试验(IPGTT),以了解移植胰岛的存活及其功能情况。结果与对照组相比,实验组受体的血糖明显下降(P<0.01),外周血C肽水平升高(P<0.01),其体质量增加(P<0.01),IPGTT结果显示其糖耐量得到改善。结论人外周血内皮细胞与胰岛共移植可明显改善裸鼠移植胰岛的生存。

Objective To explore the endothelial cells from human peripheral blood and islet of rat co-transplan- tation under the renal capsule of diabetic nude mice to improve the survival and function of transplanted islet. Methods The endothelial cells from human peripheral blood （5 - 105） and freshly isolated rat islet cells were co-transplanted under the renal capsule of diabetic nude mice model, then the fasting blood glucose, body weight, peripheral blood C-peptide level, and intraperitoneal glucose tolerance test （IPGTT） were measured to evaluated the islet graft survival and function. Results Compared with the control group, the fasting blood glucose level significantly decreased （P〈0. 01）, peripheral blood C-peptide level rised （P〈0. 01）, and body weight increased （P〈0. 01） of receptor nude mice in experience group, the IPGTT also improved. Conclusion The endothelial cells from human peripheral blood and islet of rat co-transplan- tation can obviously improve the survival and function of transplanted islet of nude mice.