肝细胞癌术后复发相关机理研究以及潜在治疗药物预测

Exploring The Mechanism of Postoperative Recurrence of Hepatocellular Carcinoma and Predicting The Candidate Drugs

目的探讨肝细胞癌(hepatocellular carcinoma,HCC)术后复发的分子机理,同时寻找预测抑制术后复发的治疗药物。方法利用35例复发与41例未复发患者的基因表达谱数据,寻找术后复发相关差异表达基因,并进行基因本体(gene ontology,GO)与生物学通路(pathway)富集分析,寻找相关的生物学功能与通路,同时利用Connectivity Map(cmap)数据库区去预测可抑制HCC术后复发的潜在药物分子。结果发现HCC术后复发相关基因显著富集到了"黏着斑信号通路"、"MAPK信号通路"等相关通路中;同时发现了2个可能的潜在治疗药物"班布特罗"和"洛伐他汀"。结论 HCC术后复发相关基因涉及到"黏着斑信号通路"、"MAPK信号通路"等通路,且"班布特罗"以及"洛伐他汀"可能作为抑制HCC术后复发的潜在药物。

Objective To explore the mechanism of postoperative recurrence of hepatocellular carcinoma （HCC） and predicting the candidate drug. Methods The differently expressed genes of the human gene expression profiles with 35 postoperative recurrence of HCC tissues and 41 no recurrence of HCC tissues were identified. Then enriched these genes with gene ontology （GO） terms and KEGG pathway, and predicting the candidate drugs for suppress the postoperative recurrence using Connectivity Map （cmap） database. Results Several pathways such as Focal adhesion and MAPK signaling pathway were found involve in postoperative recurrence of HCC. Moreover, two candidate small molecule drugs （bambuterc,1 and lovastatin） were found may suppress and postoperative recurrence of HCC. Conclusion Focal adhesion and MAPK signaling pathway may involve in the postoperative recurrence ofHCC, bambuterol and lovastatin may candidate drugs for treat postoperative recurrence of HCC.