食物诱导型代谢综合征家兔模型的建立和评价

Establishment and Evaluation of Food Induced Model of Metabolic Syndrome in Rabbit

目的建立易于形成动脉粥样硬化性疾病的代谢综合征动物模型,探索一种与人类发病可比性高的造模方法。方法 16只家兔,随机抽取8只为对照组,喂饲普通饲料,其余为模型组,喂饲高脂高糖饲料,每周称体质量,于8、10、13、16、18周测定三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、葡萄糖(GLU)水平。实验结束时,测股动脉血压、胰岛素(INS)含量,进行主动脉硬化评分、计算动脉硬化指数,计算腹部脂肪系数、肝脏系数及心脏系数,检查心脏、主动脉、肝脏及下肢动脉组织病理。结果与对照组比较,模型组体质量、血清TC、GLU、血压、脂肪系数和肝脏系数升高;HDL-C/TC下降;主动脉粥样硬化评分、动脉硬化指数增加;胰岛素敏感指数降低(P<0.05或P<0.01)。结论食物诱导法可以形成家兔代谢综合征及动脉粥样硬化模型。

Objective To establish an animal model of metabolic syndrome which is prone to atherosclerosis. Methods Eight rabbits were randomly selected from in total of 16 rabbits as control group who were fed with normal rabbit feed. And the rest were used as model group, who were fed with high fat diet. Animals were weigh every week, and triaeylg-lyeerol （TG）, total cholesterol （TC）, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL- C）, and glucose （GLU） content were tested in the 8th, 10th , 13th, 16th and 18th week. Femoral arterial blood pressure and insulin （INS） content were assessed at the end of experimental period which is the 18＇h week; Also, aortic sclerosis rating, arterial stiffness index, abdominal fat coefficient, liver coefficient and cardiac coefficient were calculated and heart, aorta, liver and lower extremity artery pathology were examed. Results Compared with the control group, weight in the model group increased significantly; Serum levels of TC, HDL-C, blood pressure abdominal fat coefficient, liver coefficient were significantly increased in model group; the whole blood viscosity, plasma viscosity and blood pressure fat coefficient, liver coefficient, aortic sclerosis rating, arterial stiffness index, and GLU all increased significantly in model group; while HDL-C/TCHO, in- sulin sensitivity index decreased significantly in model group （P 〈 0.05 or P 〈 0.01）. Conclusion Food induced method can form metabolic syndrome and atherosclerosis in rabbit model.