mfn2和nm23在不同膀胱组织中的表达

Expression of Mitofusin-2(mfn2) and nm23 in Different Bladder Tissues

目的探讨线粒体融合蛋白-2(mfn2)和肿瘤转移抑制基因(nm23)在膀胱癌组织中的表达及与临床病理特征间的关系。方法选取65例膀胱癌标本,其中男50例,女15例。TNM分期:Ⅰ期47例、Ⅱ期10例、Ⅲ期5例、Ⅳ3例,另择正常膀胱组织、良性膀胱肿瘤标本各15例作为对照。应用免疫组化SP法检测mfn2和nm23的表达情况,并分析其在膀胱癌组织中的表达与临床病理特征之间的关系。结果膀胱癌组织中mfn2的阳性表达率均高于正常和良性膀胱组织,nm23的阳性表达率均低于正常和良性膀胱组织(均P<0.05)。膀胱癌组织中mfn2的表达在不同分化程度、TNM分期间的差异有统计学意义,在不同年龄、性别、淋巴结转移情况和临床分期间的差异无统计学意义。nm23在不同TNM分期、淋巴结转移情况和临床分期间的差异有统计学意义,在不同年龄、性别和分化程度间的差异无统计学意义。结论 mfn2在膀胱癌中高表达,mfn2与膀胱癌的发生、发展密切相关;nm23在膀胱癌中低表达,可作为预测膀胱癌转移及其预后的参考指标。

Objective To research the expression of mitofusin-2 （mfn2） and tumor metastasis suppressor genes （nm23） in bladder cancer cells and its correlation with clinical pathological feature. Methods Immunohistochemistry was used to measure the expression of mfn2 and nm23. Sixty-five cases of bladder cancer were sampled, which include fifty cases of male and fifteen cases of female. TNM stage ： Forty-seven cases were in stage I; Ten cases were in stage Ⅱ; Five cases were in stage Ⅲ; Three cases were in stage IV. Other fifteen cases were sampled from normal bladder or benign tumor of bladder as control. All cases were collected from department of pathology, affiliated Hospital of hebei union university. Results The positive expression rate of mfn2 in bladder cancer tissues was significantly higher than those in normal bladder tissues and benign tumor of bladder （X2 =32.528, P 〈 0.05） ;The positive expression rate of nm23 in bladder cancer tis- sues was significantly lower than those in normal bladder tissues and benign tumor of bladder （X2 = 19.719, P〈0.05） ; the high expression level of mfn2 in bladder cancer was associated with tumor differentiation and TNM stage （P 〈 0.05 ）, but not correlated with age, sex, lymph node metastasis and clinical grade. The low level of nm23 was associated with TNM stage, clinical grade and LN metastasis. Conclusion The positive expression rate of mfn2 was increased in bladder cancer. It indicated that there was a close relationship between mfn2 and the occurrence and development of bladder cancer;The expression rate of nm23 was decreased in bladder cancer, it may be a predictor for metastasis and prognosis of bladder cancer.