长链非编码RNA H19促进人胃癌MGC-803细胞增殖的实验研究

Promotive effect of long non-coding RNA H19 on the proliferation of gastric cancer MGC-803 cells

目的探讨长链非编码RNA H19在胃癌组织及细胞株中的表达情况及其对人胃癌细胞增殖的影响。方法采用实时定量PCR检测30例胃癌组织及其对应癌旁组织,以及胃癌细胞株MGC-803、SGC-7901和胃黏膜上皮正常GES-1细胞中H19的表达情况;分析胃癌组织H19表达与临床病理特征(年龄、性别、分化程度、肿瘤浸润深度、淋巴结转移及TNM分期)的关系。将胃癌MGC-803细胞分别转染H19特异性小干扰RNA(si-H19组)和阴性对照序列(si-NC组),分别采用四甲基偶氮唑蓝法和克隆形成实验检测两组细胞的增殖情况。结果与癌旁组织和黏膜上皮正常细胞相比较,胃癌组织和细胞株中H19的表达水平升高。胃癌组织中H19表达与年龄、性别、分化程度均无关(P>0.05),但与肿瘤浸润深度、淋巴结转移及TNM分期有关(P<0.05)。si-H19组中H19表达水平低于si-NC组,且细胞活力和细胞克隆数均低于si-NC组,以上差异均有统计学意义(P<0.05)。结论胃癌组织及细胞株中H19呈高表达,下调H19表达能显著抑制胃癌细胞的增殖能力,H19可能参与了胃癌的发生、发展。

Objective To explore the expression levels of long non-coding RNA H19 in tissues and ceils of gastric cancer, and investigate the effect of H19 in cell proliforation of gastric cancer MGC-803 cell line. Methods Real-time quantitative PCR was applied to detect the expression of H19 in 30 paired tumor tissues and corresponding adjacent normal tissues, normal epithelial cell line GES-1 and gastric cancer MGC-803, SGC-7901 cell lines. The relationship between the expressions of H19 in tumor tissues with clinicopathological characteristics（ age, gender, degree of differentiation, depth of tumor invasion, lymph node metastasis and TNM stage） was analyzed. The specific small interfering RNA for H19（ si-H19 group） or negative control sequence（ si-NC group） were transfected into MGC-803 cells. The MTr assay and clone formation experiment were employed to detect the proliferation of two groups. Results The levels of H19 in both gastric cancer tissues and cells were higher, compared with adjacent normal tissues and gastric epithelial cells. The expression of H 19 was related with depth of tumor invasion, lymph node metastasis and TNM stage （P〈0. 05）, but not with age, gender and degree of differentiation （P〉 0. 05）. Compared with si-NC group, there were lower H19 level, cell activity and cell clone number in si-H19 group with statistical significance（P〈0. 05）. Conclusion There is high expression of H19 in gastric cancer tissues and cells. Down-regulating H19 expression can inhibit gastric cancer cell proliferation, which may participate in the occurrence and development of gastric cancer.