EpCAM和Ki-67在三阴性乳腺癌原发灶及淋巴结转移灶中的表达及临床意义

Expressions of EpCAM and Ki-67 in primary lesion and metastatic lymph nodes of triple-negative breast cancer and their clinical significance

目的探讨上皮细胞黏附分子(EpCAM)和Ki-67在三阴性乳腺癌(TNBC)原发灶和淋巴结转移灶中的表达及临床意义。方法用免疫组化染色检测81例TNBC原发灶、43例淋巴结转移灶及20例癌旁正常乳腺组织中EpCAM和Ki-67的表达情况。结果 EpCAM和Ki-67在TNBC组织中的过表达率分别为74.1%和61.7%,均高于癌旁正常乳腺组织,差异有统计学意义(P<0.05);EpCAM蛋白表达与TNBC的组织学分级、淋巴结转移有关(P<0.05);Ki-67蛋白表达与组织学分级、淋巴结转移及pTNM分期有关(P<0.05);TNBC组织中EpCAM和Ki-67蛋白表达呈正相关(r=0.462,P<0.001)。在43例伴有淋巴结转移的TNBC患者中,淋巴结转移灶和原发灶中EpCAM和Ki-67蛋白均呈高表达,Ki-67在淋巴结转移灶中的表达率高于原发灶(P<0.05)。结论 EpCAM和Ki-67在TNBC原发灶及淋巴结转移灶中均高表达;EpCAM和Ki-67过表达可能与TNBC的发生、发展密切相关。

Objective To investigate the expressions of epithelial cell adhesion mole-cule（EpCAM） and Ki-67 in primary le- sion and metastatic lymph nodes of triple-negetive breast cancer（TNBC） and their clinical significance. Methods The expressions of EpCAM and Ki-67 were examined by SP immunohistochemical stain（IHC） in 81 cases of TNBC, 43 cases of metastatic lymph nodes and 20 cases of normal breast tissues. Results The overexpression rates of EpCAM and Ki-67 were 74. 1% and 61.7%, respectively. Moreover, the overexpression of EpCAM and Ki-67 in TNBC was significantly higher than that in normal breast tissues（ P〈0. 05）. The overexpression of EpCAM was correlated with differentiation grade and lymph node metastasis of TNBC （P〈0.05）. On the other hand, the high expression of Ki-67 was correlated with differentiation grade, lymph node metastasis and pTNM staging of TNBC （P〈0.05）. The overexpression of EpCAM in metastatic lymph nodes was as high as in primary lesion of TNBC, but the high expression of Ki-67 in lymph node metastasis was higher than that in primary lesion（P〈0. 05）. Conclusion EpCAM and Ki-67 are expressed highly both in in primary lesion and metastatic lymph nodes of TNBC. The overexpression of EpCAM and Ki-67 can be closely related with the carcinogenesis and progression of TNBC.