乙肝相关性肝移植受者术前树突状细胞表型、功能的研究

The study of immune phenotype and function of dendritic cells from hepatitis B virus correlated liver transplant recipients before operation

目的 观察乙肝相关性肝移植受者术前体外培养的单核细胞来源的树突状细胞（DC）免疫表型、功能变化.方法 通过DC生成培养基体外诱导健康人（对照组）和拟行肝移植的乙肝患者（患者组）DC,并分别负载乙肝表面抗原（HBsAg）和乙肝核心抗原（HBcAg）诱导成熟,比较两组DC形态、表型、刺激淋巴细胞的增殖能力及培养上清中白细胞介素（IL）-12、IL-10分泌量的变化,并检测患者组中负载HBsAg和HBcAg抗原成熟的DC（负载组,n=9）与未负载抗原成熟的DC（未负载组,n=9）诱导辅助性T细胞（Th）极化的能力.结果 患者组和对照组的CD83阳性率分别为（53.10±16.23）％、（81.91±9.20）％ （P ＜0.01）,人类白细胞DR抗原（HLA-DR）及CD40/CD80/CD86等共刺激信号分子阳性率比较差异无统计学意义（P＞0.05）.患者组DC刺激自体T细胞增殖能力降低（P＜0.05）.患者组IL-12、IL-10浓度分别为（141.94±58.81）、（12.86±5.72） ng/L,与健康组比较差异无统计学意义（P＞0.05）.未负载组与负载组诱导T细胞极化（Th1）比例分别为（7.59±2.62）％、（11.17±1.11）％（P＜0.05）.结论 乙肝相关性肝病肝移植患者术前DC的表型、功能受到一定的抑制,但在体外有效的负载抗原后亦能有效的诱导Th1应答,从而激活细胞免疫,提高患者主动免疫力.

Objective To investigate the immune Phenotype and function of monocyte-derived dendritic cell （DCs） cultured in vitro from hepatitis B virus （HBV） correlated liver transplant （LT） recipients who were in the situation before operation.Methods The DCs of healthy control group and HBV correlated LT recipients before operation were induced by DC culture medium respectively,and then incubated with hepatitis B virus surface antigen （HBsAg） and hepatitis B virus core antigen （HBcAg） to induce matured DCs.Compared the DC morphology and Phenotype and Proliferative capacity and the concentration of Interleukin （IL）-12 and IL-10 in supernatants in each group （control group n =7,patient group n =9）,Meanwhile compared the capacity of DCs induced polarization of T cell between incubated group and non-incubated group.Results The rate of CD83 was （53.10 ± 16.23） in patient group,（81.91 ± 9.20） in control group respectively （P 〈0.01）.Meanwhile,the difference among HLA-DR and CD40/ CD80/CD86 were not significant （P 〉 0.1）.The proliferative capacity of DC were reduced in LT recipients （P 〈0.05）.There was no significant difference in the concentration of IL-12 and IL-10 were （141.94 ± 58.81） ng/L and （12.86 ± 5.72） ng/L in patient group respectively.The rate of Th1 induced by DCs was （7.59 ± 2.62） in non-incubated group,and （11.17 ± 1.11） in incubated group respectively （P 〈 0.05）.Conclusion The immune phenotype and function of DCs were inhibited in LT recipients before operation,but it could induce Th1 response efficiently after incubated with HBsAg and HBcAg in vitro,and increased active immunity.