乳腺浸润性导管癌组织中转录因子Twist和缺氧诱导因子-1α表达与上皮-间充质转化的关系

Expressions of Twist and hypoxia inducible factor-1α in invasive ductal carcinoma of breast and their relationships with epithelial-mesenchymal transition

目的 探讨转录因子Twist、缺氧诱导因子（HIF）-1α在乳腺浸润性导管癌组织中的表达及其与上皮-间充质转化的关系.方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶（SP）法检测90例乳腺浸润性导管癌组织及其对应癌旁组织标本中Twist、HIF-1α、E-钙黏蛋白（E-cadherin）及波形蛋白（Vimentin）的表达.结果 在乳腺癌组织中Twist、HIF-1α、E-cadherin和Vimentin的阳性率分别为73.3％、68.9％、15.6％和64.4％,癌旁组织中的阳性率分别为7.8％、8.9％、75.6％和34.4％.该4种蛋白在肿瘤组织中的表达率与癌旁组织之间差异均有统计学意义（P＜0.05）.Twist与HIF-1α的表达均与组织学分级和腋窝淋巴结转移有关（P＜0.05）.Twist与HIF-1α的表达呈正相关（P＜0.05）.Twist、HIF-1α的表达也均与E-cadherin的低表达和Vimentin的高表达密切相关（P＜0.05）.结论 Twist、HIF-1α和Vimentin在乳腺癌组织中呈高表达,而E-cadherin呈低表达.HIF-1α可能通过上调Twist表达,促进上皮-间充质转化发生,成为乳腺癌浸润转移的途径之一.

Objective To investigate the expression of Twist and hypoxia inducible factor-1α （HIF-1α） in invasive ductal carcinoma of breast and their relationships with epithelial-mesenchymal transition （EMT）.Methods Expression levels of Twist,HIF-1α,E-cadherin and Vimentin in 90 cases of invasive ductal carcinoma tissues and corresponding adjacent tissues were detected by immunohistochemical staining of streptavid-in-peroxidase （SP） method.Results The positive expression rate of Twist,HIF-1 α,E-cadherin and Vimentin was 73.3%,68.9%,15.6% and 64.4% respectively in breast cancer tissues,and 7.8%,8.9%,75.6% and 34.4% respectively in adjacent tissues.The difference in positive rate between breast cancer tissues and adjacent tissues was statistically significant （P 〈 0.05）.The expression levels of Twist and HIF-1α were correlated with histological grade and lymph node metastasis （P 〈 0.05）.There was a positive correlation between expression levels of Twist and HIF-1α.The expression levels of Twist and HIF-1α were closely correlated with low expression of E-cadherin and high expression of Vimentin.Conclusion Twist,HIF-1α and Vimentin in breast cancer tissues showed higher expression,while E-cadherin lower expression.HIF-1α may promote the occurrence of EMT by up-regulating the expression of Twist,which makes it become one of the possible ways to promote the invasion and metastasis of breast cancer.