橙皮苷防龋作用的动物实验研究

The effects of hesperidin in caries prevention in rats

目的:研究橙皮苷在大鼠体内的防龋作用。方法:纸片扩散法检测橙皮苷对远缘链球菌(S.sobrinus)的最小抑菌浓度(MIC),接种S.sobrinus建立大鼠龋病模型,分别用1/2 MIC橙皮苷、0.12%洗必泰、无菌蒸馏水处理大鼠口腔,菌落计数法动态监测S.sobrinus水平、Keyes龋齿评分法、激光龋齿诊断仪(DIAGNOdent)探测荧光值评价不同处理方法对大鼠龋病发生的影响。结果:橙皮苷的MIC为8 mg/ml;S.sobrinus水平:橙皮苷组与蒸馏水组相比差异无统计学意义(P>0.05);Keyes记分:橙皮苷组光滑面及窝沟E级龋损记分低于蒸馏水组(P<0.05),橙皮苷组窝沟Ds级、Dm级龋损记分低于蒸馏水组(P<0.05),高于洗必泰组(P<0.05);DIAGNOdent探测荧光值:橙皮苷组龋损程度低于蒸馏水组(P<0.05),高于洗必泰组(P>0.05)。结论:1/2 MIC橙皮苷在不影响大鼠口内菌群水平的前提下,可减少大鼠磨牙龋病形成及减轻龋损程度。

Objective：To investigate the anticaries effects of hesperidin in rats.Methods：The MIC of hesperidin against Streptococ-cus sobrinus（S.sobrinus）was explored with disc diffusion method.The rats with artificial caries were administered with 1/2 MIC hes-peridin,0.12%Chlorhexidine and distilled water respectively.The results of S.sobrinus level,Keyes scoring and DIAGNOdent exam-ination were used to evaluate the effects of hesperidin on S.sobrinus and caries development.Results：The MIC of hesperidin agaist S. sobrinus was 8 mg/ml.The S.sobrinus level was not statistically different between hesperidin group and negative control group（P〉0. 05）.Keyes scores of grade E of smooth and pit and fissure caries in hesperidin group were lower than in negative control（P〈0.05）, those of grade Ds and Dm of pit and fissure caries in hesperidin group were lower than in negative control（P〈0.05 ）,while higher than in chlorhexidine group（P〈0.05）.Hesperidin showed significant anti-caries effect（P〈0.05）examined by DIAGNOdent.Con-clusion：Hesperidin at 1/2 MIC has anti-caries effect in rats without influence of the oral microecological balance.