HU-308缓释涂层对大鼠成骨细胞生物学特性的影响

The effects of sustained-release coating of HU-308 on the biological characteristics of rat osteoblasts

目的:探讨HU-308缓释涂层对大鼠成骨细胞(ROBs)生物学特性的影响。方法:利用层层自组装技术在经碱热处理后的钛片表面制备含不同浓度HU-308的肝素/壳聚糖缓释涂层。以碱热处理的钛片为对照组(C组),肝素/壳聚糖涂层组(P组)及含不同浓度HU-308的肝素/壳聚糖涂层组(T组:T1、T2、T3、T4组)为实验组,在不同时间点,检测ROBs在涂层表面的黏附、增殖以及ALP活性。结果:T4组涂层(HU-308浸渍浓度为0.025 mg/L)表面ROBs的黏附、增殖以及ALP活性均强于P组及C组。结论:低浓度HU-308缓释涂层可提高ROBs在其表面的黏附、增殖和ALP活性。

Objective：To observe the the effects of sustained-release coatings of HU-308 on the biological characteristics of rat oste-oblasts（ROBs）on Ti surface.Methods：The heparin/chitosan sustained release coatings containing HU-308 at different concentra-tions were prepared by layer-by-layer assembly technology on the alkali-and heat-treated Ti surface.The alkali-and heat-treated Ti samples were randomly divided into six groups,the samples in C group were used as control,in P group were treated by heparin/chi-tosan coatings and in T1,T2,T3 and T4 groups were treated by heparin/chitosan sustained-release coating containing HU-308 at dif-ferent concentrations.The adhesion,proliferation and ALP activity of ROBs cultured on the coatings were assessed at different times. Results：The adhesion,proliferation and ALP activity of ROBs of T4 group （the impregnating concentration of HU-308 was 0.025 mg/L）were the highest.Conclusion：The sustained-release coating with low concentration of HU-308 may improve the adhesion , proliferation and ALP activity of ROBs.