摘要
目的:观察外源性的血小板衍生生长因子-BB(rhPDGF-BB)与转化生长因子-β1(rhTGF-β1)联合应用对大鼠正畸牙压力侧破骨细胞FAK mRNA基因水平的表达变化。方法:将160只SD大鼠随机分为实验组和对照组。每组又根据检测时间点不同分为5个小组(n=16),分别建立正畸牙移动模型。实验组从加力第1天开始在正畸牙颊侧黏膜下隔日注射rhPDGF-BB 10 ng与rhTGF-β15 ng,对照组注射相同容量的PBS。加力后1、4、7、10和14 d分别处死每大组的一小组大鼠。收集标本,用TRAP染色观察压力侧破骨细胞数量的变化,实时定量PCR(RT-PCR)检测FAK mRNA基因的表达。结果:rhPDGF-BB及rhTGF-β1联合注射明显促进压力侧破骨细胞数目的增加(P<0.05);破骨细胞内FAK mRNA基因量的表达7 d前增加(P<0.05),7 d后逐渐降低,14 d时与对照组无显著差异(P>0.05)。结论:外源性PDGF-BB和TGF-β1联合应用促进正畸牙压力侧破骨细胞增殖,增加了破骨细胞内FAK mRNA基因表达。
Objective：To study the effects of recombinant human platelet-derived growth factor-BB（rhPDGF-BB）combined with recombinant human transforming growth factor-β1 （rhTGF-β1 ）on the expression of FAK mRNA of osteoclasts in the alveolar bone on the pressure side of orthodontic teeth in rats.Methods：Orthodontic tooth movement model was established in 160 male SD rats.The rats in experimental group were treated by injection of 10 ng rhPDGF-BB and 5 ng rhTGF-β1 in the buccal submucosal area of the mo-lar every other day from day 1 afterburner,while those in control group received equivalent volumes of PBS.The rats were sacrificed at 1,4,7,10 and 14 days（n=16）after appliance placement.Specimens were collected.Osteoclasts in the alveolar bone on the pres-sure side of the orthodontic teeth were observed by TRAP staining,the FAK mRNA expression was quantified by quantitative RT-PCR.Results：rhPDGF-BB combined with rhTGF-β1 significantly promoted an increasing number of osteoclasts on the compressing side（P〈0.05）,increased the expression of FAK mRNA at day 4 and 7（P〈0.05）,then decresed it to the control level（P〉0.05） at day 14.Conclusion：Combination of rhPDGF-BB and rhTGF-β1 can increase the number of osteoclasts in the alveolar bone on compressing side,and promote FAK mRNA expression in osteoclasts.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2014年第5期619-623,共5页
Journal of Practical Stomatology
基金
贵州省科学技术基金[编号:黔科合J字LKZ(2011)05号]
