牙龈卟啉单胞菌感染对血管内皮细胞与单核细胞黏附的影响

The effects of Porphyromonas gingivalis infection on mononuclear cell adhesion to vascular endothelial cells

目的:研究Pg感染对人脐静脉内皮细胞(HUVEC)与单核细胞黏附的影响。方法:建立Pg感染HUVEC细胞株EA.hy926的体外模型,虎红染色法观察Pg381和Pg33277菌株感染HUVEC 6 h和24 h后,HUVEC与人单核细胞株THP-1细胞黏附量的变化。结果:培养6 h时Pg381感染组的THP-1细胞黏附量相对值为0.210±0.025,高于Pg33277感染组(0.078±0.024,P<0.05)和空白对照组(0.062±0.022,P<0.05),Pg33277感染组和空白对照组无差异。24 h时3组间无差异(P>0.05)。结论:Pg381感染HUVEC后能显著促进其与单核细胞的黏附。

Objective：To elucidate the effects of Pg in modulation of monocyte adhesion to human umbilical vein endothelial cells （HUVECs）.Methods：The model of infection of HUVECs by Pg was established in vitro.HUVECs were infected with Pg381 and Pg33277 and then incubated with THP-1 monocytes respectively.The amount of THP-1 adhesion to HUVECs was tested by rose Ben-gal staining assay.Results：Pg381-infected HUVECs demonstrated significantly increased adhesion of THP-1 cells compared to those infected with Pg33277（0.210 ±0.025 vs 0.078 ±0.024,P〈0.05）or control HUVECs（0.062 ±0.022,P〈0.05）after 6 h cul-ture.There was no differences among the above three groups after 24 h culture（P〉0.05）.Conclusion：The infection of Pg381 can increased the adhesion of monocytes to HUVECs.