人工合成甘蓝型油菜S_0-S_3代DNA及cDNA的AFLP分析

AFLP Analysis on DNA and cDNA in S_0-S_3 Generation of Artificially Synthetic Brassica napus

为研究人工合成甘蓝型油菜早期世代基因组及表达水平的遗传变异规律,本研究利用AFLP(扩增片段长度多态性)和cDNA-AFLP分子标记技术,以人工合成甘蓝型油菜的亲本、S0代、S3代植株为材料,用26对引物进行扩增。结果表明:S3世代在基因组水平共发生了6.33%的变异,其中发生在A基因组和C基因组上的变异条带分别占总扩增条带的1.68%和1.81%,不确定变异条带占总扩增条带的2.84%;S3世代在表达水平共发生了81.34%的变异,其中发生在A基因组和C基因组的变异条带分别占总扩增条带的16.36%和21.20%,30条变异条带来源于AC共有条带,占总扩增条带的6.91%;不确定变异条带占总扩增条带的36.87%。本研究结果说明人工合成甘蓝型油菜S3代基因组水平和表达水平均发生了一定程度的变异,其中表达水平的变异率较高,此外,基因组水平和表达水平的变异均倾向于发生在C基因组上。

In order to analyse the genetic variation of genome and expression levels of Artificially Synthetic Brassica napus in the early generations, the DNA and cDNA of the parents, S0 and S3 generations of the Artifi- cially Synthetic Brassica napus were examined with 26 pairs of AFLP （amplified fragment length polymorphism） primer pairs. The results showed that 6.33% variation of genome occurred in S3-generation, which accounted for 1.68% and 1.81% of total amplified bands on A and C genomes, respectively. The bands, whose the variation origin were uncertain, accounted for 2.84% of total amplified bands; 81.34% variation was observed on cDNA level in S3-generation, The variation bands from the A and C genomes accounted for 16.36% and 21.20% of total amplified bands, respectively. 30 variation bands were from shared bands by A and C genomes, accounting for 6.91% of total amplified bands. The bands, whose the variation origin were uncertain, accounted for 36.87% of total amplified bands. As a whole, the results of this study show that the Artificially Synthetic Brassica nopus＇s S3-generation occurred a certain degree of variation in genome level and cDNA level, with the cDNA level occurring higher variation rate. Furthermore, the variation of genome level and expression level tended to occur on the C genome.