小桐子根腐病拮抗芽孢杆菌的筛选

Screening of the Antagonistic Bacillus on Root Rot of Jatropha curcas

小桐子镰刀菌根腐病的生物防治尚未见报道。为筛选小桐子根腐病镰刀菌拮抗芽孢杆菌,本研究从15份健康植株根际土壤分离细菌69株,从5份根腐病植株根际土壤分离细菌14株,筛选出12株高拮抗细菌并鉴定出8株枯草芽孢杆菌、2株芽孢杆菌、2株短短芽孢杆菌,其中枯草芽孢杆菌B2-1抑菌圈直径达21 mm。结果表明,枯草芽孢杆菌B2-1抑菌圈直径大、生长速率高,具有小桐子根腐病镰刀菌的潜在生防价值;但田间防效受物理、化学、微生物种群等因素的影响,有待进一步研究。

The biological control of Fusarium sp. of Jatropha curcas has not been reported. In order to screen anta- gonistic bacillus on Fusarium sp. of Jatropha curcas, 69 strains bacteria were isolated from 15 healthy soil and 14 strains from 5 rhizosphere soil infected by Fusarium sp., subquencely 12 antagonistic strains among them were selected and identified as 8 strains Bacillus subtilis, 2 strains Bacillus sp. and 2 strains Brevibacillus brevis. Especially, there are antagonistic zone width up to 21.0 mm and quicker growth rate for Bacillus subtilis B2-1. The results show that Bacillus subtilis B2-1 has potential biocontrol value because of its diameter of inhibition is big, the growth rate is high. The effectiveness in field was influenced by physical factors, chemical factors, mocrobial population and so on, which needs be studied further.