2013年河南省新布尼亚病毒的分离与S片段基因特征分析

Novel bunyavirus and its S segment genome in Henan Province,China,2013

目的 分离2013年河南省新布尼亚病毒分离株,并了解S片段序列特征.方法 采集2013年的发热伴血小板减少综合征急性期血清,用Vero细胞分离新布尼亚病毒,对分离到的毒株采用RT PCR法扩增病毒S片段基因,并进行同源性分析,构建系统进化树.结果 从采集的64份标本中分离到新布尼亚病毒22株,新分离的毒株的S片基因序列比对发现,同源性在94.6％～100％之间,其双义编码的两个蛋白质,NSs蛋白的氨基酸的同源性在95.90％～100％之间,N蛋白的氨基酸的同源性在99.50％～100％之间.系统进化树分析发现,22株病毒株分属于A、B和E三个基因型,其中A型18株,B和E型各2株.结论 2013年河南省新布尼亚病毒以A基因型为主要流行型别.

To isolate the novel bunyavirus in Henan Province, China, 2013, acute phase serums from severe fever with thrombocytopenia （SFTS） patients were cultured using Vero cells. S segment complete sequences were amplified by RT-PCR from the positive cultured samples, and sequence homology and phylogenetic analysis were carried out. As a result, 22 novel bunyavirus strains were isolated from 64 SFTS acute phase serum in Henan, 2013, in which the sequence identity of complete S segment was from 95.90% to 100%, and that of encoding nucleocapsid protein was from 99.50% to 100%. Twenty-two strains belonged to A, B and E genotypes, including 18 strains of type A and 2 strains of B and E in each, which suggested a genotype of novel bunyavirus prevailed in Henan Province in the year of 2013.