醒脑静注射液对酒精性昏迷大鼠外侧下丘脑区(LHA)谷氨酸和γ-氨基丁酸的影响

Effect of Xingnaojing Injection on glutamate and GABA in lateral hypothalamic area of alcoholic coma rat

目的:研究醒脑静注射液对酒精性昏迷大鼠外侧下丘脑区(LHA)谷氨酸和γ-氨基丁酸的影响。方法:腹腔注射乙醇制作酒精性昏迷大鼠模型,20min后侧脑室给药,微透析技术收集透析样品(每30 min收集一次),OPA柱前衍生化法处理样品,高效液相色谱(Synergi Hydro-RP C18,150 mm×4.6 mm,4μm),荧光检测脑脊液中谷氨酸(Glu)、γ-氨基丁酸(GABA)的含量。结果:与模型对照组比较,醒脑静注射液0.34 mg/kg、0.68 mg/kg、1.37 mg/kg外侧下丘脑区(LHA)Glu、GABA恢复到正常水平的时间更短。结论:醒脑静注射液能够通过调节酒精性昏迷大鼠外侧下丘脑区(LHA)Glu、GABA的含量,使其恢复到正常水平从而促进觉醒。

To study the effect of Xingnaojing Injection on glutamate and GABA in lateral hypothalamic area of alcoholic coma rat. Methods： Producing alcoholic coma rat models by intraperitoneal injection and intracerebroventricular administration 20 minutes later, sam- ples were collected by microdialysis （lateral hypothalamic area, collected every 30 minutes）. O-phthaldialdehyde was used for the derivatiza-tion of the samples, then HPLC-FLU（ Synergl Hydro-RP C18,150 mm × 4.6mm,4 μm）was used to measure the levels of glutamate and GA- BA in dialysis fluid. Results： Comparing with the model control group, the levels of glutamate and GABA in lateral hypothalamic area of ex- perimental group（0.34 mg/kg,0. 68 mg/kg, 1.37 mg/kg）was found to be recover in a shorter time. Conclusions： Xingnaojing Injection could promote arousal of alcoholic coma rats by modulating the content of glutamate and GABA in LHA to normal level.