运动点电针对急性脊髓损伤大鼠半胱氨酸天冬氨酸蛋白酶3和降钙素基因相关肽表达的影响

The impact of electroacupuncture at motor points on caspase-3 and calcitonin gene related peptide expression of rats with acute spinal cord injury

目的:探讨运动点电针对急性脊髓损伤大鼠半胱氨酸天冬氨酸蛋白酶3(caspase-3)和降钙素基因相关肽(CGRP)表达的影响。方法:将60只SD大鼠随机分为4组,每组15只。A组:运动点电针组;B组:非运动点电针组;C组:模型组;D组:假手术组。采用改良的Allen法建立大鼠急性脊髓损伤模型。A、B组分别于术后立即开始下肢运动点和非运动点进行电针治疗;C、D组不做任何治疗。各组大鼠分别于1、4、7d行BBB评分,通过免疫组织化学方法(IHC)观察各组caspase-3和CGRP阳性细胞的表达情况。结果:60只SD大鼠均进入结果分析。BBB评分结果显示:A组评分明显高于B、C组(P<0.05);免疫组化结果显示:1caspase-3的表达:损伤后各组均可见阳性细胞表达,D组仅见少量的阳性细胞。其中A、B组阳性细胞表达明显低于C组(P<0.05);而A组的表达又明显低于B组(P<0.05);2CGRP的表达:各组均可见阳性细胞表达,A、B组明显高于C组(P<0.05),而A组又明显高于B组(P<0.05)。结论:运动点电针可抑制caspase-3和促进CGRP的表达,可能有助于脊髓功能的恢复。

Objective： To discuss the impact of electroacupuncture at motor points on caspase-3 and calcitonin gene related peptide（CGRP） expressions of rats with acute spinal cord injury. Method： Sixty SD rats with spinal cord injury were randomly divided into four groups, 15 rats in each group：group A： electroacupuncture at motor points; group B： electroacupuncture at non-motor points; group C： model group; group D： sham-operated group. Modified Allen＇s method was used to establish rats model of acute spinal cord injury. Rats in groups A and B acupuncture therapy were administered at motor points and non-motor points immediately after spinal cord injury. Rats in groups C and D received no treatment. At day 1,4 and 7, BBB（Basso-Beattie-Bresnahan） scores were assessed in each group,Then the expressions of caspase-3 and CGRP positive cells of rats after acute spinal cord injury in each group were observed by immunohistochemistry method. Result： BBB scores：In group A BBB scores were significantly higher than that in groups B and C（P〈0.05）. Immunohistochemical results：①Caspase-3： The expressions of positive cells emerged in all groups after injury, in group D only small amount of positive cells emerged.The expressions of positive cells in groups A and B were significantly lower than that in group C （P〈0.05）,and the expression of positive cells in group A was lower than that in group B （P〈0.05）.②CGRP：The expressions of positive cells emerged in all groups. The expressions of positive cells in groups A and B were significantly higher than that in group C（P〈0.05）, and the expression of positive cells in group A was higher than that in group B （P〈0.05）. Conclusion： Electroacupuncture at motor points can suppress the expression of caspase-3 and promote the expression of CGRP and would be helpful for the spinal cord function recovery.