Notch基因在变应性鼻炎小鼠模型中的表达及其意义

Expression and significance of Notch receptors in the mouse model of allergic rhinitis

目的：探讨Notch信号通路相关基因在变应性鼻炎模型小鼠鼻黏膜及外周血单个核细胞（PBMC）中的表达及意义。方法：7～8周龄BALB/c小鼠16只,随机分成对照组和模型组。以卵清蛋白作为过敏原,建立变应性鼻炎小鼠模型,分析比较小鼠过敏症状评分,采用苏木精-伊红染色分析比较鼻黏膜病理变化及炎性细胞的浸润程度,采用酶联免疫吸附法检测并分析小鼠外周血血清中IgE的表达水平。荧光定量聚合酶链反应和流式细胞术从不同水平检测模型组小鼠鼻黏膜及PBMC中Notch信号通路相关受体（Notch1～Notch4）的表达量,并与对照组比较。结果：成功建立了变应性鼻炎BALB/c小鼠模型。模型组小鼠鼻黏膜中Notch1、Notch3、Notch4mRNA的表达明显高于对照组（P〈0.01）,而Notch2mRNA的表达却低于对照组（P〈0.05）;与此一致,模型组小鼠PBMC中Notch1、Notch3、Notch4的蛋白表达也明显高于对照组（P〈0.01）,而Notch2的蛋白却低于对照组（P〈0.05）。结论：Notch基因的表达在变应性鼻炎模型小鼠发病过程中有显著改变,可能参与变应性鼻炎的发生发展,为深入研究变应性鼻炎的发病机制提供了思路。

Objective：To investigate the expression and significance of related receptors of Notch signaling pathway in mouse model of allergic rhinitis（AR）.Method：Sixteen BALB/c mice of seven-eight weeks old were randomly assigned to two groups,including controls group and model group.AR model mice was sensitized with ovalbumin（OVA）.Symptom score,hematoxylin-eosin for pathological alteration and infiltration of inflammatory cells in nasal mucosa were analyzed as well as enzyme linked immunosorbent assay was taken to detect IgE in peripheral serum.Nasal septum mucosa and peripheral blood mononuclear cells were collected from 16BALB/c mouse（8Allegic rhinitis,8controls）.Notch 1-4were checked by real-time quantitative polymerase chain reaction and flow cytometry from different levels.Result：BALB/c mice model of allergic rhinitis was established successfully.The mRNA of Notch1,Notch3,Notch4 in nasal septum mucosa of allergic rhinitis mice model groups were obviously higher than that in normal controls,and the difference were statistically significant（P〈0.01）.However,The expression of Notch2 is lower than the control group and the difference was statistically significant（P〈0.05）.In line with the above,the protein expression of Notch1,Notch3,Notch4 in peripheral blood mononuclear cells（PBMC）of model groups were significantly higher than that in health controls,and the difference were statistically significant（P〈0.01）.But comparing control,expression of Notch2 was lower and the difference was statistically significant（P〈0.05）.Conclusion：There were significant changes of Notch genes in mouse model of AR.This intimated that related genes of Notch signaling pathway may paly important roles in the development and progression of AR and provide ideas for in depth study of the pathogenesis of AR.