PPAR-γ在CRSsNP鼻窦黏膜和CRSwNP鼻息肉中表达差异性研究

Expression of PPAR-γ in patients with chronic rhinosinusitis without or with nasal polyps

目的:探讨过氧化物酶体增殖物激活受体-γ(PPAR-γ)在慢性鼻-鼻窦炎不伴鼻息肉(chronic rhinosinusitis without nasal polyps,CRSsNP)患者鼻窦黏膜和慢性鼻-鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)患者鼻息肉中的表达差异及临床意义。方法:应用实时定量PCR技术和免疫组织化学技术检测PPAR-γ在CRSsNP鼻窦黏膜、CRSwNP鼻息肉及正常下鼻甲中的表达。结果:1PPAR-γ在CRSsNP鼻窦黏膜、CRSwNP鼻息肉及正常下鼻甲中均有表达;2PPAR-γ的mRNA和蛋白水平在CRSsNP鼻窦黏膜及CRSwNP鼻息肉中的表达较正常下鼻甲组织下降,差异均有统计学意义(P<0.05);3PPAR-γ的mRNA和蛋白水平在CRSsNP鼻窦黏膜和CRSwNP鼻息肉中的表达无显著性差异。结论:PPAR-γ在人的鼻腔黏膜中有表达,同时在CRSsNP鼻窦黏膜及CRSwNP鼻息肉中的表达下降。PPAR-γ可能与CRSsNP及CRSwNP炎症长期存在有关,提示激活PPAR-γ可能为慢性鼻-鼻窦炎治疗提供一种新的方法。

Objective：To investigate the expression of peroxisome proliferator-activated receptorγ（PPAR-γ）in the sinus mucosa of chronic rhinosinusitis without nasal polyps（CRSsNP）and chronic rhinosinusitis with nasal polyps（CRSwNP）.Method：Using immunohistochemistry and RT-PCR,testing the content of PPAR-γin sinus mucosa and nasal polyps of patients with chronic sinusitis without nasal polyps and normal inferior turbinate,and analyzing the differences.Result：①The PPAR-γwas found both in sinus mucosa of CRSsNP,nasal polyps of CRSwNP and in normal inferior turbinate;②The expression of PPAR-γin sinus mucosa of CRSsNP and nasal polyps of CRSwNP was lower than that in normal inferior turbinate tissue,and the differences was prominent in statistics;③No differences in the expression of PPAR-γwere obtained in sinus mucosa of CRSsNP and nasal polyps of CRSwNP.Conclusion：The PPAR-γexpressed in human nasal mucosa,meanwhile it was descending in sinus mucosa of CRSsNP and nasal polyps of CRSwNP.The long-term inflammations resulted from CRSsNP and nasal polyps of CRSwNP might relate to PPAR-γ,it suggested that PPAR-γagonists may offer a new therapy for the treatment of Chronic Rhinosinusitis.