异硫氰酸苯乙酯对胃癌细胞体外抑制作用相关研究

Effect of PEITC on gastric cancer cells in vitro

[目的]研究异硫氰酸苯乙酯(PEITC)体外实验中对人胃癌BGC-823细胞的增殖抑制作用及诱导其凋亡相关分子机制。[方法]应用MTT法、Annexin V/PI双染法检测PEITC人胃癌BGC-823细胞增值以及凋亡的影响;应用RT-PCR以及Western Bolt检测PEITC诱导胃癌细胞凋亡相关基因蛋白的表达情况。[结果]PEITC作用人胃癌细胞BGC-823给药24h后,与空白组比较,能显著抑制胃癌细胞的增殖,抑制作用与浓度和时间成依赖关系,诱导胃癌细胞的凋亡,能上调Bax表达,下调Bcl-2、Bcl-xl、Survivin等基因表达,激活Caspase-3、Caspase-9、PARP活性。[结论]PEITC能有效抑制人胃癌BGC-823细胞的增殖,其机制可能是通过激活Caspase家族蛋白酶活性而诱导胃癌细胞凋亡。

[Objective] To study the inhibition effect of phenethyl isothiocyanate（PEITC）on human gas-tric cancer BGC-823 cell growth in vitro and the related molecular mechanism of inducing apoptosis. Fmeth-ods^MTT and Annexin V/PI double staining method were adopted for detection of proliferation and apop-tosis in human gastric cancer BGC-823 cells. RT-PCR and Western blot were used to detect PEITC inducedapoptosis in gastric cancer cells and inhibition of invasion and adhesion. EResults3Compared with the blankgroup,the growth of gastric carcinoma cells BGC-823 treated by PEITC for 24 h were remarkably inhibi-ted,presenting a concentration and time dependent manner. PEITC also induced apoptosis in gastric cancercells, upregulated the expression of Bax, downregulated Bcl-2, Bcl-xl, survivin gene expression, activatedCaspase-3,Caspase-9,and PARP activity. Econclusion PEITC can inhibit human gastric cancer cell BGC-823,which may be realized by inducing apoptosis of gastric cancer cells through activating caspase familyproteases activity.