自拟涤痰汤对非酒精性脂肪肝大鼠血脂代谢及肝功能的影响

Effects of self-prepared Ditan decoction on blood lipid metabolism and liver function in rats with non-alcoholic fatty liver disease

目的:观察涤痰汤对非酒精性脂肪肝大鼠的影响。方法:将100只实验大鼠随机分为正常对照组,模型组,涤痰汤高、低剂量组和易善复组,对除正常对照组外的其余4组大鼠均喂食高脂饲料6周制备非酒精性脂肪肝大鼠模型。实验第2周起,各治疗组大鼠分别给予涤痰汤高、低剂量及易善复灌胃,连续给药5周。实验结束后检测比较各组大鼠体质量、肝重、血清学指标、肝脏组织匀浆指标。结果:与正常组相比,模型组大鼠体质量、血清TC、TG、AST、ALT水平及肝组织匀浆TC、TG水平均较正常组明显升高,表明脂肪肝造模成功;与模型组相比,涤痰汤高、低剂量组和易善复组均能降低模型大鼠血清TC、TG、AST、ALT水平及肝脏组织匀浆TC、TG水平,差异均有统计学意义(P<0.05)。结论:涤痰汤可改善非酒精性脂肪肝模型大鼠血清血脂水平,并能改善肝脏脂肪变性。

Objective：To observe the effects of Ditan decoction on the blood lipid metabolism and liver function in rats with non - alcoholic fatty liver disease （NAFLD）. Methods：One hundred experimental rats were randomly divided into normal control group, model group, high - and low - dose Ditan decoction groups, and Yishanfu group. All rats except those in the normal control group were fed high - fat diet for 6 weeks to establish a rat model of NAFLD. From the second week of the experiment, all treatment groups were given high - or low - dose Ditan decoction or Yishanfu by gavage for 5 weeks. At the end of experiment, all groups were compared in terms of body weight,liver weight, serological parameters, and liver tissue homogenate indices. Resuhs ：Compared with the normal control group, the model group had significantly increased body weight, serum levels of total cholesterol （ TC ）, triglyceride （ TG ）, aspartate amin- otransferase （AST）, and alanine aminotransferase （ALT）, and TC and TG levels in liver tissue homogenate, indicating that the fatty liv- er model was successfully established. Compared with the model group, the high - and low - dose Ditan decoction groups and Yishanfu group had significantly reduced serum levels of TC, TG, AST, and ALT and levels of TC and TG in liver tissue homogenate （ P 〈 0. 05 ）. Conclusion：Ditan decoction can reduce serum lipid levels and liver fatty degeneration in rats with NAFLD.