加味苇茎汤及其成分芹菜素对肺腺癌A549细胞增殖、周期与凋亡的影响

Inhibitory Effect of Self-made Jiawei Weijing Decoction and Apigenin on Proliferation,Cell-cycle and Apoptosis of Lung Adenocarcinoma A549 Cell

探讨加味苇茎汤含药血清及成分芹菜素对肺腺癌A549细胞增殖、细胞周期及凋亡的影响,选用肺癌A549细胞体外培养,在不同浓度含药血清及芹菜素作用下,用MTT法检测细胞生长抑制率、流式细胞术检测细胞周期及凋亡率、hoechst33342染色检测细胞凋亡形态。结果显示,加味苇茎汤含药血清及芹菜素对A549的生长抑制率:24 h分别为31%、34%,48 h分别为45%、53%;流式细胞术检测细胞周期发现A549细胞经加味苇茎汤含药血清处理后主要阻滞在G2期,而芹菜素能明显引起细胞凋亡。荧光显微镜下观察到较典型的细胞凋亡形态甚至细胞脱落,视野内细胞核数明显减少。该研究结果显示,加味苇茎汤含药血清及芹菜素能明显抑制A549细胞的增殖,引起细胞周期阻滞、诱导其凋亡。

The objectives of this study were to investigate the inhibitory effect of self-made Jiawei Weijing Decoction and apigenin on the prollferation,ceIl cycle and apeptosis of lung adenocarcinoma A549 cell llne and to explore its mecha- nism in the treatment of lung cancer. The experimental study used an inverted microscope observation of cell morpholo- gy. MTY assay was performed to reveal the inhibitory effect on cell proliferation and flow cytometry was used to determine the apotosls and cell cycle of lung adenocarcinoma A549 cell line after the treatment of self-wade Jiawei Weijing Decoc- tion and apigenin. Morphological changes of the apeptosis of cancer cells were observed by hochest 33342 staining. The results showed that compared with controls, the inhibition rates of self-made Jiawei Weijing decoction and apigenin on lung adenocarcinoma A.549 cells were： in 24 hours group,31% and 34% ,respectively; in 48 hours group,45% and 53% ,respectively. Self-made Jiawei Weijing decoction increased the rate of G2 of A549 cell cycle obviously and apige- nin significantly induced the apoptosis of A549 cell. Hence,it was concluded that Self-made Jiawei Weijing decoction and apigenin inhibited the growth of human lung adenocarcinoma A549 cells and cause cell cycle arrest and induce ap- optosis.