羧甲基壳聚糖金属配合物的抗氧化活性研究

Antioxidant Activity of Carboxymethyl Chitosan Metal Complexes

研究了壳聚糖、羧甲基壳聚糖及3种羧甲基壳聚糖金属配合物(CMC-Cu、CMC-Co、CMC-Ag)的抗氧化活性,同时考察了金属离子含量对CMC-Cu配合物抗氧化活性的影响。结果表明:对羟自由基的清除能力依次为CMC-Cu>CMC-Co>CMC-Ag>羧甲基壳聚糖>壳聚糖,清除能力随着浓度的增大而增强;对超氧负离子自由基清除能力依次为CMC-Ag>CMC-Cu>CMC-Co>羧甲基壳聚糖>壳聚糖,清除能力随浓度的增加而增强;还原能力依次为CMC-Co>CMC-Ag>CMC-Cu>羧甲基壳聚糖>壳聚糖。Cu2+含量与CMC-Cu配合物的抗氧化活性有关。

In this study, the antioxidant activities of carboxymethyl chitosan metal complexes were evaluated using hy- droxyl free radical scavenging assay, superoxide anion free radical scavenging assay and reducing power assay. The re- sults showed that all of the metal complexes exhibited the antioxldant activity in different levels. The order of carboxym- ethyl chitosan metal complex＇ s scavenging effect on hydroxyl free radical was CMC-Cu 〉 CMC-Co 〉 CMC-Ag 〉 car- boxymethyl chitosan 〉 chitosan;the order carboxymethyl chltosan metal complex＇ s scavenging effect on superoxide ani- on free radical was CMC-Ag 〉 CMC-Cu 〉 CMC-Co 〉 carboxymethyl chitosan 〉 chltosan, and the order of carboxymeth- yl chitosan metal complex＇ s reducing power was CMC-Co 〉 CMC-Ag 〉 CMC-Cu 〉 carboxymethyl chitosan 〉 chi- tosan. The content of Cu2 ＋ was related to antioxidant activity of CMC-Cu.