人参、刺五加提取物对链脲佐菌素诱导糖尿病小鼠的降糖作用研究

Hypoglycemic Effect of Extract from Panax ginseng and Acanthopanax senticosus on Streptozotocin-induced Diabetic Mice

本实验通过观察高血糖小鼠空腹血糖(Fasting blood glucose,FBG)、糖耐量、血清胰岛素敏感指数(Insulin sensitivity index,ISI)、血清超氧化物歧化酶(Superoxide dismutase,SOD)和丙二醛(Malondialdehyde,MDA)含量变化,探讨人参与刺五加配伍药物降糖的初步机制。采用腹腔注射链脲佐菌素(Streptozotocin,STZ)的方法建立小鼠糖尿病模型,根据随机分组对照法,对各组小鼠进行不同干预处理。结果表明,给药组小鼠空腹血糖和糖耐量均显著降低(P<0.01或P<0.05),血清胰岛素敏感性及SOD活性显著回升(P<0.01或P<0.05),血清MDA含量明显下降(P<0.01或P<0.05)。这表明人参与刺五加配伍可有效改善糖尿病小鼠空腹血糖水平并增强其抗氧化能力,同时提示其降糖机制可能与增强胰岛素敏感性,提高抗氧化能力等有关。

The objective of this study was to observe the hypoglycemie effect of ethanol extract from Panax ginseng and Acarahopanax sentlcosns on diabetic mice induced by streptozotocin （ST＇Z） ,and to explore its preliminary hypoglyeemic mechanism. In this experiment fasting injection of STZ was used to establish the mouse model d diabetes. Randomized control method was used to divide mice into different groups,inclodes 4 of hlgh-dose groups （400 mg/kg） ,4 of low-dose groups （200 mg/kg） ,glibenclamide group,model group and normal control group. Each group was ig administrated with different concentrations of ethanol extract of P. ginseng and 75% ethanol extract of A. senticosns for 6 consecutive weeks. The contents of body weight, fasting blood glucose （FBG）, glucose tolerance, serum Insulin levels and antioxidant levels were assayed after the last ig administration. The results showed that compared with those of the normal control group, FBG levels of model group increased significantly （P 〈0.01） ,body weigh decreased;compared with those of the model group, FBG levels, glucose tolerance of high-dose groups and low-dose groups were significantly lower than the model group （P 〈0.01 or P 〈0.05） ,insulin sensitivity and antioxidant activity were improved. In conclusion,P, ginseng com- bined with A. senticosns can effectively improve fasting blood glucose levels of diabetic mice. However,it was found that 50% ethanol extract of P. ginseng with 75% ethanol extract of A. senticosus showed the most significant hypoglycemic effects. The mechanism was possibly through improving antioxidant levels, increasing ISI and improving the insulin sensi- tivity in hyperglycemic.