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快速敲除补回家蚕核型多角体病毒ORF29

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摘要 利用Red重组系统成功敲除家蚕核型多角体病毒ORF29基因(BmNPV ORF29,简称Bm29),并利用Bac-to-Bac系统构建补回型的Bm29基因。通过PCR鉴定敲除和补回均成功,为后续的Bm29基因功能研究奠定基础。
作者 王蓉
机构地区 浙江理工大学
出处 《浙江农业科学》 2014年第10期1624-1626,共3页 Journal of Zhejiang Agricultural Sciences
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参考文献15

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