王草砖红壤土壤根际解磷菌的分离鉴定及培养条件研究

Study on isolation, identification and culture condition of rhizosphere phosphate-solubilizing microorganisms from red paddy soil of king grass

从王草（Cynodon dactylon L.）根际砖红壤土壤中筛选到21株解磷菌,对其形态学和生理生化特性进行了测定,最后将筛选出的解磷能力较强且无拮抗反应的4株解磷细菌组合成为解磷菌群,对其最适温度、最适pH值和最适生长时间进行了研究。结果表明,菌株2-5、2-6、2-8-1、2-9、3-8、3-8-1、4-1-2、4-2、4-5为巨大芽孢杆菌（Bacillus megaterium）;菌株2-7-1、2-8、3-6-1、4-1-1、4-3为伯克霍尔德菌属（Burkholderia sp）;菌株4-4、2-7、2-8-2、3-6、4-3-1为蜡样芽孢杆菌（Bacillus cereus）。解磷菌群的最适生长温度为25-30℃,最适生长pH值为6.5-7.0,最适生长时间为90 h。

Twenty-one strains of Phosphate-solubilizing bacteria were screened from red paddy soil samples of king grass, and physiological and biochemical characters of these strains were determined. Finally, 4 phosphate-solubilizing bacterial strains with stronger phosphate-solubilizing ability but without antagonism were screened, and combined as flora of phosphate-solubilizing bacteria, then their optimum temperature, optimum initial pH and optimum growth time were studied. The results showed that Strains 2-5, 2-6, 2-8-1, 2-9, 3-8, 3-8-1, 4-1-2, 4-2, 4-5 were Bacillus megaterium,Strains 2-7-1, 2-8, 3-6-1, 4-1-1, 4-3 were Burkholderia sp., Strains 4-4, 2-7, 2-8-2, 3-6, 4-3-1 were Bacillus cereus.The optimum temperature for phosphate-solubilizing bacteria flora was 25℃-30℃, the optimum initial pH was 6.5-7.0 and the optimum growth time was 90 h.