罗非鱼养殖池塘底泥微生物群落代谢功能的动态变化

Dynamics of metabolic activities of microbial communities in the sediment of Tilapia culture ponds

利用传统的平板培养法和Biolog ECO微平板法调查了罗非鱼养殖过程池塘底泥异养细菌数量和微生物群落代谢功能的动态变化。结果表明:底泥异养细菌数量在整个养殖过程中均较稳定,其波动高峰出现在6月份,波动范围为(8.4±5.7)×106^(5.2±4.2)×107CFU/g;池塘底泥微生物群落代谢活性和功能多样性以在各个月份之间的差异性均不显著。此外,还分析了底泥微生物群落的碳源代谢特征,底泥微生物群落对4大类碳源的利用,各月份之间无显著性差异;对不同种类碳源的利用存在差异,对糖类的利用率最高,对氨基酸、脂肪酸、代谢中间产物和次生代谢物的利用率较低。

The amount of heterotrophic bacteria and metabolic activities of microbial communities in the sediment of Tilapia culture ponds were investigated by using plate count method and Biolog ECO microplate. The results showed that the amount of heterotrophic bacteria in the sediment was stable in the whole farming period, ranging between（8.4±5.7） ×10^6and（5.2±4.2） ×10^7CFU/g and peaking in June, and the differences in metabolic activities and functional diversities of the microbial communities were not significant among each month. The characteristics of carbon metabolism of the microbial communities were also analyzed. The results showed that there was no significant difference among each month in the utilization of four kinds of carbon sources. However, there were significant differences in the utilization of different carbon sources, higher rate was found for the utilization of saccharides, and low rate was found for the utilization of amino acids, fatty acids, metabolic intermediates and secondary metabolites.