摘要
目的:建立青鹏软膏中麝香酮的含量测定方法。方法:采用气相色谱法检测,样品用无水乙醇超声提取后进样,采用HP-INNOWax Polyethylene Glycol毛细管色谱柱(30.0 m×320μm×0.25μm),柱温:梯度升温(0~15 min:110℃、16~30 min:以10℃/min上升),火焰离子化检测器(FID)检测麝香酮的含量,进样分流比为20∶1。结果:方法的标准曲线方程为A=587.18C-13.7,r=0.999 7,线性范围为0.2~0.8mg/mL;平均回收率为99.5%(n=9),RSD为0.9%。结论:本方法准确、专属、灵敏度高,操作简便、快速,适用于青鹏软膏中麝香酮的含量测定。
Objective: To establish content determination method ofmuscone in QingPeng ointment. Methods: Gas chromatography was adopted, and the sample was prepared by ultrasonic extraction of absolute ethyl alcohol, HP-INNOWax Polyethylene Glycol capillary chromatographic column (30.0 m×320μm×0.25μm), and column tem perature: gradient heating-up (0-15 min: 110℃, 16-30 min: ascending at the rate of 10℃/min), flame ionization detector (FID), sample split ratio: 20:1, detected by external standard method. Results: Standard curve equation of the method was: A=587.18C-13.7, r=0.999 7, linear range: 0.2-0.8 mg/mL; average recovery rate was 99.5% (n=9), RSD was 0.9%. Conclusion: The method, accurate, specific, sensitive, simple and rapid, could be used for content determination ofmuscone in QingPeng ointment.
出处
《西部中医药》
2014年第9期53-55,共3页
Western Journal of Traditional Chinese Medicine
关键词
青鹏软膏
麝香酮
气相色谱法
QingPeng ointment
muscone
gas chromatography