气相色谱法测定藏药青鹏软膏中麝香酮的含量

Content Determination of Muscone in Tibetan QingPeng Ointment by Gas Chromatography

目的：建立青鹏软膏中麝香酮的含量测定方法。方法：采用气相色谱法检测,样品用无水乙醇超声提取后进样,采用HP-INNOWax Polyethylene Glycol毛细管色谱柱（30.0 m×320μm×0.25μm）,柱温：梯度升温（0~15 min：110℃、16~30 min：以10℃/min上升）,火焰离子化检测器（FID）检测麝香酮的含量,进样分流比为20∶1。结果：方法的标准曲线方程为A=587.18C-13.7,r=0.999 7,线性范围为0.2~0.8mg/mL;平均回收率为99.5%（n=9）,RSD为0.9%。结论：本方法准确、专属、灵敏度高,操作简便、快速,适用于青鹏软膏中麝香酮的含量测定。

Objective： To establish content determination method ofmuscone in QingPeng ointment. Methods： Gas chromatography was adopted, and the sample was prepared by ultrasonic extraction of absolute ethyl alcohol, HP-INNOWax Polyethylene Glycol capillary chromatographic column （30.0 m×320μm×0.25μm）, and column tem perature： gradient heating-up （0-15 min： 110℃, 16-30 min： ascending at the rate of 10℃/min）, flame ionization detector （FID）, sample split ratio： 20：1, detected by external standard method. Results： Standard curve equation of the method was： A=587.18C-13.7, r=0.999 7, linear range： 0.2-0.8 mg/mL; average recovery rate was 99.5% （n=9）, RSD was 0.9%. Conclusion： The method, accurate, specific, sensitive, simple and rapid, could be used for content determination ofmuscone in QingPeng ointment.