芍药苷和芍药苷亚硫酸酯代谢产物的比较

Comparative study of paeoniflorin and paeoniflorin sulfite metabolites

目的:应用高效液相色谱法比较芍药苷和芍药苷亚硫酸酯的代谢产物。方法:将2组健康Wistar大鼠分别按500 mg·kg-1灌胃给予芍药苷和芍药苷亚硫酸酯,于给药前及给药后5,15,30,45,60,90,120,180,240 min由眼眶静脉丛采血,血样经预处理后注入高效液相色谱进行分析。结果:芍药苷组不出现芍药苷亚硫酸酯的峰,芍药苷的体内浓度大约在45 min达到最大值,未知代谢产物X在45 min左右达到最大值;芍药苷亚硫酸酯组也会出现芍药苷的峰,该峰在30 min左右达到最大值,芍药苷亚硫酸酯峰和未知代谢产物X均在30 min左右达到最大值,但此组在给药3 h时已经检测不到芍药苷。结论:大鼠在给予芍药苷亚硫酸酯单体后在体内会部分转化为芍药苷,然而,芍药苷亚硫酸酯不会完全转化为芍药苷,应该适当控制用硫磺熏蒸芍药。

OBJECTIVE To compare the metabolic products of paeoniflorin and paeoniflorin sulfite by HPLC. METHODS Two groups of healthy Wistar rats were administrated with paeoniflorin and paeoniflorin sulfite individually, blood collection from the orbital venous plexus before and after drug administration in 5, 15, 30, 45, 60, 90, 120, 180, 240 min. RESULTS Paeoniflorin group did not appear paeoniflorin sulfite peaks. Paeoniflorin concentration reached a maximum in vivo at about 45 min after drug administration, the unknown metabolite X also reached a maximum at 45 min. The peak of paeoniflorin appeared in paeoniflorin sulfite group, which reached a maximum at about 30 min after drug administration, paeoniflorin sulfite peak and the unknown metabolites were about 30 min reached the maximum, but paeoniflorin could not detect 3 h after administration of paeoniflorin sulfite. CONCLUSION Rats given paeoniflorin sulfite is partially transformed into paeoniflorin in vivo, however, paeoniflorin sulfite can not completely convert into paeoniflorin, fumigate of Chinese herbaceous peony with sulfur should be properly controlled.