高效液相色谱法测定生远志及其炮制品中远志皂苷B的含量

Determination of onjisaponin B in Radix Pologala and processing products by HPLC

目的：建立远志药材中远志皂苷B的测定方法。方法：采用高效液相色谱法,色谱柱为Agilent TC-C18色谱柱（4.6mm×250 mm,5μm）,流动相为乙腈-0.1%磷酸水溶液（38∶62）,检测波长307 nm,柱温：30℃,流速：1.0ml·min-1。结果：回归方程为：Y=10.865 X＋220.16（r=0.999 8）,线性范围为1.778-17.78μg,平均回收率（n=6）为99.24%（RSD=2.4%）。5个产地药材中远志皂苷B含量为1.62%-2.26%;蜜制远志中远志皂苷B的含量明显下降,甘草汁炒远志、酒炙远志、炒远志含量则变化不大。结论：该方法操作简便、准确度高、重复性好,能够对远志及其炮制品中远志皂苷B进行含量测定,为提高远志药材的质量控制标准提供了另一个有效的方法。

OBJECtIVE To establish a HPLC method for determination of onjisaponin B in Polygala. METHODS The determination was performed on an Agilent TC-C18 chromatographic column（4. 6 mm × 250 mm, 5 μm） with acetonitrile-0. 1% phosphoric acid as the mobile phase（38：62）. The detection wavelength was 307nm, the column temperature was set at 30 ℃ and the flow rate was kept at 1.0 ml·min-1. RESULTS The regression equation of onjisaponin B was Y= 10. 865X＋ 220. 16（r = 0.999 8）and the linear range was 1. 778 - 17.78 μg. The average recovery（n = 6） of onjisaponin B was 99.24% （RSD = 2. 4%）. The contents of onjisaponin B were I. 62% - 2. 26% in Radix Polygala from different regions. The contents of onjisa- ponin B in honey Polygala significantly reduced by comparing with raw Polygala and small change in the other processed prod- ucts. CONCLUSION This method is simple, accurate, reproducible, can be applied to the quality control of onjisaponin B in polygala as well as processed products, which supplies another effective method for advance the quality control of polygala.