超高效液相色谱与高效液相色谱法对比测定金银花药材中木犀草苷的含量

Comparison of UPLC and HPLC for the determination of luteolin-7-glu in Lonicera japonica Thunb

目的:比对UPLC与HPLC法测定金银花药材中木犀草苷的含量,优化测定金银花中木犀草苷含量的方法。方法:建立UPLC和HPLC测定金银花药材中木犀草苷的含量方法。UPLC法为Agilent ZORBAX RH C18(2.1 mm×50 mm,1.8μm),流动相采用乙腈-0.1%磷酸水溶液梯度洗脱,流速0.4 ml·min-1,柱温30℃,检测波长350 nm;HPLC法色谱柱为Agilent ZOTBAX XDB-Phenyl(4.6 mm×250 mm,5μm),流动相乙腈-0.4%冰醋酸溶液梯度洗脱,流速1.0ml·min-1,柱温30℃,检测波长350 nm。结果:2种方法所得含量测定结果一致。HPLC法和UPLC法加样回收率的RSD分别是1.83%和1.24%。结论:UPLC法在缩短分析时间的同时又得到更高的分析灵敏度,大大减少了有机溶剂的消耗,因此UPLC法能够替代HPLC法对金银花中木犀草苷的含量进行测定。

OBJECTIVE To compare the method of UPLC and HPLC for determination of luteolin-7-glu in Lonicera japoni- ca Thunb. METItODS UPLC and HPLC methods were established for the determination of luteolin-7-glu in Lonicera japonica Thunb. UPLC： The chromatographic separation was performed on a Agilent ZORBAX RH C18 （2. 1 mm× 50 mm, 1.8 μm） column with the mobile phase consisting of 0. 4 % phosphoric acid and methanol, gradient elution separation. The flow rate was 0. 4 ml.min-1. HPLC： The chromatographic separation was performed on an Agilent ZOTBAX XDB-Phenyl （4. 6 mm × 250 mm, 5 μm）column, the mobile phase consisted of 0. 4 ~ acetic acid and methanol, gradient elution separation. The flow rate was 1.0 ml-min-1. The detection wavelength was 350 rim, the column temperature was 30 ℃. RESULTS Both methods for the determination had the similar results. The RSD of the average recovery of UPLC and HPLC was 1.24% and 1.83%, respec- tively. CONCLUSION UPLC can not only increase the separation speed and efficiency, but also reduce the analysis time and solvent consumption. UPLC instead of HPLC method for the determination of luteolin-7-glu in Lonicera japonica Thunb. is simple, the results are accurate and reliable.