去铁胺对老龄大鼠学习记忆能力的影响

Effect of deferoxamine on learning and memory ability in aged rats

目的 评价去铁胺对老龄大鼠学习记忆能力的影响.方法 清洁级健康雄性SD大鼠42只,18月龄,体重450 - 550 g,采用随机数字表法,将其分为2组（n=21）：生理盐水组（N组）和去铁胺组（D组）.D组腹腔内注射甲磺酸去铁胺150 mg/kg,1次/d,连续6d.N组以等容量（2 ml）生理盐水替代.于当日给药后2h开始行Morris水迷宫实验,共进行6d,记录逃避潜伏期、游泳速度、目标象限停留时间、中心区域停留时间.于首次给药前、第3天、第6天给药后2h,采用Western blot法检测海马铁蛋白表达.结果 与N组比较,D组逃避潜伏期缩短,目标象限停留时间百分比和中心区域停留时间百分比升高,海马铁蛋白表达下调（P＜0.05）,游泳速度差异无统计学意义（P＞0.05）.结论 去铁胺可增强老龄大鼠的学习记忆能力,其机制与减少海马内铁沉积有关.

Objective To evaluate the effect of deferoxamine on learning and memory ability in aged rats.Methods Forty-two healthy male Sprague-Dawley rats,aged 18 months,weighing 450-550 g,were randomly divided into 2 groups （n =21 each） using a random number table：normal saline group （group N） and deferoxamine group （group D）.In group D,deferoxamine 150 mg/kg was injected intraperitoneally once a day for 6 consecutive days,while the equal volume of normal saline was given instead in group N.Morris water maze test was conducted at 2 h after each injection on that day,lasting for 6 days.The escape latency,swimming speed,time of staying at the original platform quadrant and time spent in the central region were recorded.Hippocampal ferritin expression was detected by Western blot before the first administration and at 2 h after 3rd and 2nd administration.Results Compared with group N,the escape latency was significantly shortened,and the percentage of the time of staying at the original platform quadrant and time spent in the central region was increased,the expression of hippocampal ferritin was down-regulated,and no significant change was found in the swimming speed in group D.Conclusion Deferoxamine can enhance the learning and memory ability in aged rats,and reduced iron deposition in hippocampi is involved in the mechanism.