肿节风酸性多糖的纯化及其对α-葡萄糖苷酶的抑制作用

Purification of an acidic polysaccharide from Sarcandra glabra and its inhibitory effect on α-glycosidase activity

制备肿节风酸性粗多糖(SGP-C),采用优化的纯化条件(pH为9,H2O2浓度5%,温度50℃,时间3.5 h)获得SGP-O,再经过Sephacryl S-400分子筛纯化获得纯品SGP-2(纯度大于98%),得率为0.35%。测定了SGP-2对α-葡萄糖苷酶活性的影响。结果表明,SGP-2具有较强的α-葡萄糖苷酶抑制作用,IC50为(87.06±11.76)μg/mL,仅为阿卡波糖IC50(338.9±46.86μg/mL)的25%。初步动力学研究表明其抑制类型属于混合型可逆抑制。

Through the preparation of the crude polysaccharide （SGP-C） from Sarcandra glabra （Thunb）Nakai, the polysaccharide of SGP-O was obtained with optimal purification conditions （ pH 9, 5% H202, 50 ℃,decolored for 3.5 h）. Then, high-quality polysaccharide （SGP-2） was purified by Sephacryl S-400 molecularsieve with the yield of 0.35%. In vitro oL-glucosidase inhibition assay indicated that SGP-2 had an IC50 of （ 87.06 ±11.76） μg/mL, only a quarter of that of acarbose （ 338.9 ±46.86 Ixg/mL）, which indicated that SGP-2 hadstrong a-glucosidase enzyme inhibitory activity. Preliminary dynamic studies showed that SGP-2 inhibited oL-glu-cosidase in a reversible and mixed-type mode.