普洱市2012年手足口病病原监测分析

Monitoring and analysis of hand-foot-mouth disease pathogen in 2012 Puer city

目的了解普洱市2012年手足口病的病原体情况,为手足口病的防控和治疗提供实验室依据。方法采集本市各级医疗机构临床诊断为手足口病患者的咽拭子和疱疹液标本,提取病毒核酸,采用Real-time RT-PCR法,检测肠道病毒71型(EV71)和柯萨奇病毒A16型(CoxA16)。结果 2012年共检测552份手足口病病人标本,肠道病毒核酸阳性284份,阳性率51.45%,CoxA16阳性率为29.71%(164/552),EV71阳性率为21.74%(120/552)。结论 2012年普洱市手足口病病原以EV71和CoxA16为主,5岁以下儿童是肠道病毒感染的高危人群,儿童肠道病毒感染无明显性别差异。

Objective To learn the hand- foot- mouth disease pathogenof Puer city in 2012,and provide basis for prevention,control and treatment hand- foot- mouth disease. Methods Collect of hand- foot- mouth disease clinical diagnosis of pharyngeal swab and herpes fluid specimens from medical institutions at all levels in this municipality,extract the virus nucleic acid by the Real- time RT- PCR method,and detect enterovirus 71（ EV71） and coxsackie virus A16（ CoxA16）. Results In2012,a total of 552 HFMD patients specimens were detected,284 intestinal virus nucleic acid was positive,positive rate of51. 45%,CoxA16 positive rate was 29. 71%（ 164 /552）,EV71 positive rate was 21. 74%（ 120 /552）. Conclusion 2012 Puer city of hand- foot- mouth disease pathogen is given priority to with EV71 and CoxA16,children under the age of five is enterovirus infection of high- risk groups,and no obvious gender difference was found among children with enterovirus infection.