重庆市生牛乳中金黄色葡萄球菌的污染状况及耐药性调查的研究

Survey and study on the pollution and drug resistance of golden staphylococcus in fresh milks of Chongqing

目的了解生牛乳中金黄色葡萄球菌污染状况及其耐药性,预防食源性疾病的发生。方法 2012年收集重庆市各地奶站、养殖场及奶牛养殖户150份,生牛乳按GB4789.10-2010方法进行检测,并对检出的金黄色葡萄球菌作药物敏感试验。结果 150份生牛乳样品共检出金黄色葡萄球菌37株,检出率为24.67%(37/150)。金黄色葡萄球菌对9种抗生素出现不同程度的耐药,以青霉素为最高,达89.19%,四重以上耐药菌株有13株,达35.14%。结论生牛乳中金黄色葡萄球菌污染程度高,耐药及多重耐药严重。必须加强生牛乳卫生管理及生产过程的消毒管理,控制抗生素的使用,建立相应的食源性疾病的监测系统,严防食源性疾病的发生。

Objective To learn the pollution and drug resistance of golden Staphylococcus in fresh milks so as to reduce the dangerous disease caused by foodborne pathogens. Methods 150 fresh milk samples collected from milk station,farm,and dairy farmers for isolating Staphylococcus and detected by GB4789. 10- 2010 and make drug sensitivity tests of the golden Staphylococcus. Results There were 37 strains of Staphylococcus among the 150 samples and the detection rate was 24. 67%（ 37/150）,Drug resistances occurred on 9 antibiotics among 13 antibiotics especially to penicillin,reaching to 89.19%. More than quadruple of drug- resistant strains was 13 strains,reaching to 35. 14%. Conclusion The pollution of Staphylococcus in fresh milk was to a high degree and drug resistance as well as multiple drug resistance was serious. Milk hygiene and the process sterilizing in the production process must be strengthened,the using of antibiotics must be controlled and monitoring system is to established to prevent the foodborne disease.