摘要
目的：建立微生态活菌制品地衣芽孢杆菌活菌颗粒活菌数的测定方法。方法：稀释液为灭菌生理盐水,采用营养琼脂平皿涂布法37℃培养18~24 h。结果：该方法在2~326 CFU·皿-1范围内菌落计数结果与预期菌落计数呈良好的线性关系,线性方程为Y=1.0082X-1.8532（r=0.9997）;回收率为99.5%（n=9）。结论：本方法经方法验证,结果准确可靠,重复性好,适用于地衣芽孢杆菌活菌颗粒的质量控制。
Objective：To establish a method for live bacteria counting of Bacillus licheniformis particles. Methods： The sterilized saline was used as a diluent. Bacillus licheniformis was spread and grew on nutrient agar at 37 ℃for 18 -24 hours. Results：This method showed a good linear relationship （ Y = 1. 0082X - 1. 8532, r = 0. 9997 ）for count- ing results with the expected count in the range of 2 -326 CFU · plate-1. And the recovery was 99. 5% （n =9）. Conclusion ：The methodology validation showed that the method was accurate, reliable, reproducible, and suitable for the quality control of viable Bacillus licheniformis particles.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2014年第10期1792-1795,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
微生态活菌制品
地衣芽孢杆菌
活菌颗粒
活菌数测定
涂布法
方法学验证
Key words： Micro ecological living bacterium products
Bacillus licheniformis
viable particles
live bacteria count-ing
coating method
methodology validation
