摘要
目的:建立同时测定柏药灌肠液中盐酸小檗碱和乌药醚内酯的HPLC方法。方法:采用ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈(A)-0.1%磷酸溶液(B)为流动相,梯度洗脱(0~5 min,40%A;5~10 min,40%A→60%A;10~15 min,60%A;15~20 min,60%A→40%A),流速1.0 mL·min-1,检测波长235 nm,柱温30℃。结果:盐酸小檗碱和乌药醚内酯的线性范围分别为21.20~212.0μg·mL-1(r=0.9995)和10.72~107.2μg·mL-1(r=0.9997);回收率(n=6)分别为98.8%和98.6%,RSD分别为1.4%和1.6%。结论:该方法灵敏度高,专属性强,可用于柏药灌肠液的质量控制。
Objective: To establish an HPLC method for the simultaneous determination of berberine hydrochloride and linderane in Baiyao rectal solution. Methods: A ZORBAX SB - C18 column(250 mm × 4. 6 ram,5 μm) was used, and the mobile phase was acetonitrile (A) - 0. 1% phosphoric acid (B) and eluted with a gradient ( 0 - 5 min, 40%A;5 - 10 rain,40% A→60% A; 10 - 15 min,60% A; 15 - 20 rain,60% A--~40% A). The flow rate was 1.0 mL · min - 1, the detection wavelength was set at 235 nm, and the column temperature was maintained at 30 ℃. Results: The linear ranges of berberine hydrochloride and linderane were 21.20 -212.0 μg· mL-1 (r = 0. 9995 ) and 10. 72 -107.2μg · mL-1 (r =0. 9997) ,respectively;the mean recoveries were 98.8% and 98.6% with RSDs of 1.4% and 1.6% ,respectively. Conclusion:The established method is sensitive and specific,which can be used for quality control of Baiyao rectal solution.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2014年第10期1864-1867,共4页
Chinese Journal of Pharmaceutical Analysis