单因素考察和正交试验结合优选黄芪药材蛋白质提取工艺

Optimization Investigation of Extraction Technology of Protein in Astragali radix by Single Factor Test and Orthogonal Test

目的:优选黄芪药材可溶性蛋白质的最佳提取工艺。方法:对黄芪药材蛋白质提取条件进行单因素考察,并采用L9(34)正交试验设计法,采用考马斯亮蓝染色法,以黄芪药材可溶性蛋白质含量为指标,考察料液比、pH值、提取温度、提取时间四个因素对可溶性蛋白质提取率的影响,从而确定黄芪药材蛋白质的最佳提取工艺。结果:建立了黄芪药材蛋白质的最佳提取工艺:向黄芪药材粉末中加入20倍量pH为12的蒸馏水,在温度30℃条件下回流提取10 min。结论:优化的提取工艺稳定、合理、可行,能正确反映黄芪药材中的蛋白质含量,为黄芪生物活性物质的进一步研究提供了实验依据。

Objective：To optimize the extraction technology of protein in Astragali radix. Methods：The optimized extraction technology was investigated by using single factor test and orthogonal test with the content of water - soluble protein as the index for the first time which was determined by the method of coomassie brilliant blue staining. In this study, the effect of solid - liquid ratio, pH, temperature and time on the extraction rate was investigated. Results ： The optimized extraction technique of protein in As- tragali radix was established, that was pH 12, solid - liquid ratio 1 ： 20 and 10 min for extraction at the temperature of 30 ℃. Con- clusion：The optimal extraction condition was stable and reliable, and could provide an experimental basis for further study of the bioactive substance of Astragali radix.